miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
8879 3' -56.2 NC_002484.1 + 44491 0.71 0.412807
Target:  5'- uACGGCCaggaCGugGGCGGCGccgucagcuaccgaGACGGCCc -3'
miRNA:   3'- -UGUCGGg---GUugCUGCUGUa-------------CUGCCGG- -5'
8879 3' -56.2 NC_002484.1 + 48929 0.71 0.400004
Target:  5'- aACAGCUUCAucugGCG-CGACAUGGUGGUCa -3'
miRNA:   3'- -UGUCGGGGU----UGCuGCUGUACUGCCGG- -5'
8879 3' -56.2 NC_002484.1 + 49531 0.68 0.580784
Target:  5'- gACAGCCCUggacaauccgAACGucCGGCGUGcuucCGGUCa -3'
miRNA:   3'- -UGUCGGGG----------UUGCu-GCUGUACu---GCCGG- -5'
8879 3' -56.2 NC_002484.1 + 50800 0.71 0.400004
Target:  5'- ---uUCCCGugGACGACcUGGcCGGCCu -3'
miRNA:   3'- ugucGGGGUugCUGCUGuACU-GCCGG- -5'
8879 3' -56.2 NC_002484.1 + 51010 0.67 0.633872
Target:  5'- uCGGCCUCAAgcuUGGCGAUGUacgcggcGACGGCg -3'
miRNA:   3'- uGUCGGGGUU---GCUGCUGUA-------CUGCCGg -5'
8879 3' -56.2 NC_002484.1 + 51461 0.69 0.524495
Target:  5'- uACAGCCCUucagcuGCGACGACccagcagGACGauccgucugguuccGCCa -3'
miRNA:   3'- -UGUCGGGGu-----UGCUGCUGua-----CUGC--------------CGG- -5'
8879 3' -56.2 NC_002484.1 + 55452 0.7 0.437241
Target:  5'- -gAGCCCauaAACGuCGACAUcaGAUGGCUc -3'
miRNA:   3'- ugUCGGGg--UUGCuGCUGUA--CUGCCGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.