Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8879 | 3' | -56.2 | NC_002484.1 | + | 15741 | 0.67 | 0.634958 |
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Target: 5'- -aGGCCgCAGCGAUGACccca-GGCCc -3' miRNA: 3'- ugUCGGgGUUGCUGCUGuacugCCGG- -5' |
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| 8879 | 3' | -56.2 | NC_002484.1 | + | 40608 | 0.66 | 0.678301 |
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Target: 5'- gGCGcGCCUCA---ACGGCAaGACGGUCa -3' miRNA: 3'- -UGU-CGGGGUugcUGCUGUaCUGCCGG- -5' |
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| 8879 | 3' | -56.2 | NC_002484.1 | + | 9667 | 0.66 | 0.678301 |
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Target: 5'- aGCAGCCUUcuUGGCuGCGcgcuUGAUGGCCc -3' miRNA: 3'- -UGUCGGGGuuGCUGcUGU----ACUGCCGG- -5' |
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| 8879 | 3' | -56.2 | NC_002484.1 | + | 3593 | 0.66 | 0.678301 |
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Target: 5'- cCAGUUCaugAGCG-CGGCGcgGACGGCCu -3' miRNA: 3'- uGUCGGGg--UUGCuGCUGUa-CUGCCGG- -5' |
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| 8879 | 3' | -56.2 | NC_002484.1 | + | 9320 | 0.66 | 0.689056 |
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Target: 5'- cGCuGCCUgAACcACGAuCAUuGCGGCCu -3' miRNA: 3'- -UGuCGGGgUUGcUGCU-GUAcUGCCGG- -5' |
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| 8879 | 3' | -56.2 | NC_002484.1 | + | 40238 | 0.66 | 0.699757 |
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Target: 5'- aGCAGCgauaCCgCAACGGCG-C-UGAUGGCa -3' miRNA: 3'- -UGUCG----GG-GUUGCUGCuGuACUGCCGg -5' |
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| 8879 | 3' | -56.2 | NC_002484.1 | + | 28420 | 0.66 | 0.699757 |
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Target: 5'- gGCGGCCgCCGGCauucuCGGCGUGA--GCCg -3' miRNA: 3'- -UGUCGG-GGUUGcu---GCUGUACUgcCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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