Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8879 | 3' | -56.2 | NC_002484.1 | + | 36620 | 0.71 | 0.409124 |
Target: 5'- gGCGGCCCUGAucgcCGGCGGCAUGcccggggaAUGcGCCa -3' miRNA: 3'- -UGUCGGGGUU----GCUGCUGUAC--------UGC-CGG- -5' |
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8879 | 3' | -56.2 | NC_002484.1 | + | 35985 | 0.71 | 0.409124 |
Target: 5'- cGCGGCgaggaCGACGACGGCAacGCGGCUg -3' miRNA: 3'- -UGUCGgg---GUUGCUGCUGUacUGCCGG- -5' |
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8879 | 3' | -56.2 | NC_002484.1 | + | 35428 | 0.71 | 0.409124 |
Target: 5'- gAUGGCCUaguCGGCGACA--ACGGCCu -3' miRNA: 3'- -UGUCGGGguuGCUGCUGUacUGCCGG- -5' |
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8879 | 3' | -56.2 | NC_002484.1 | + | 48929 | 0.71 | 0.400004 |
Target: 5'- aACAGCUUCAucugGCG-CGACAUGGUGGUCa -3' miRNA: 3'- -UGUCGGGGU----UGCuGCUGUACUGCCGG- -5' |
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8879 | 3' | -56.2 | NC_002484.1 | + | 50800 | 0.71 | 0.400004 |
Target: 5'- ---uUCCCGugGACGACcUGGcCGGCCu -3' miRNA: 3'- ugucGGGGUugCUGCUGuACU-GCCGG- -5' |
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8879 | 3' | -56.2 | NC_002484.1 | + | 17564 | 0.72 | 0.356408 |
Target: 5'- uACGGUCa-GACGAcCGAUAccUGACGGCCa -3' miRNA: 3'- -UGUCGGggUUGCU-GCUGU--ACUGCCGG- -5' |
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8879 | 3' | -56.2 | NC_002484.1 | + | 26373 | 0.72 | 0.32401 |
Target: 5'- cCAGCUCCugauCGAUGACcgccucGGCGGCCg -3' miRNA: 3'- uGUCGGGGuu--GCUGCUGua----CUGCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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