Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8901 | 3' | -53.5 | NC_002484.1 | + | 6941 | 0.7 | 0.608172 |
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Target: 5'- gCCUGCGUa-GCGUcCGCAGCGAUcaacucgcgacccGGCCc -3' miRNA: 3'- -GGAUGCGgcCGCA-GUGUUGUUA-------------CCGG- -5' |
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| 8901 | 3' | -53.5 | NC_002484.1 | + | 26504 | 0.7 | 0.609272 |
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Target: 5'- aCCUcgacCGCgGGCGcucgcacggCACAGCAAUGGgCa -3' miRNA: 3'- -GGAu---GCGgCCGCa--------GUGUUGUUACCgG- -5' |
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| 8901 | 3' | -53.5 | NC_002484.1 | + | 46901 | 0.7 | 0.627982 |
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Target: 5'- cCCUGCguucaccgguugcaGCCGGCG-CACG-CAAUGaaaGCCg -3' miRNA: 3'- -GGAUG--------------CGGCCGCaGUGUuGUUAC---CGG- -5' |
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| 8901 | 3' | -53.5 | NC_002484.1 | + | 52148 | 0.69 | 0.653303 |
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Target: 5'- aCUUGCuggaGCCGGCGUUACA-----GGCCu -3' miRNA: 3'- -GGAUG----CGGCCGCAGUGUuguuaCCGG- -5' |
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| 8901 | 3' | -53.5 | NC_002484.1 | + | 35439 | 0.69 | 0.653303 |
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Target: 5'- gCUAuuCGCCGGauggccuaGUCgGCGACAAcGGCCu -3' miRNA: 3'- gGAU--GCGGCCg-------CAG-UGUUGUUaCCGG- -5' |
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| 8901 | 3' | -53.5 | NC_002484.1 | + | 49386 | 0.69 | 0.664288 |
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Target: 5'- uCCUGuuuUGCgGGCGUCACugcuCGAUccucccuucaGGCCa -3' miRNA: 3'- -GGAU---GCGgCCGCAGUGuu--GUUA----------CCGG- -5' |
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| 8901 | 3' | -53.5 | NC_002484.1 | + | 13465 | 0.68 | 0.707816 |
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Target: 5'- gCCgcCGCCGGgG-C-CAACAGcgGGCCu -3' miRNA: 3'- -GGauGCGGCCgCaGuGUUGUUa-CCGG- -5' |
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| 8901 | 3' | -53.5 | NC_002484.1 | + | 27373 | 0.66 | 0.828345 |
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Target: 5'- aCCUGCGCCacaccugCGCGGC-AUGGCUg -3' miRNA: 3'- -GGAUGCGGccgca--GUGUUGuUACCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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