Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8901 | 3' | -53.5 | NC_002484.1 | + | 46901 | 0.7 | 0.627982 |
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Target: 5'- cCCUGCguucaccgguugcaGCCGGCG-CACG-CAAUGaaaGCCg -3' miRNA: 3'- -GGAUG--------------CGGCCGCaGUGUuGUUAC---CGG- -5' |
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| 8901 | 3' | -53.5 | NC_002484.1 | + | 47854 | 0.68 | 0.739721 |
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Target: 5'- aCC-ACGuuGGCGcCGCGAUAccaaauuUGGCUg -3' miRNA: 3'- -GGaUGCggCCGCaGUGUUGUu------ACCGG- -5' |
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| 8901 | 3' | -53.5 | NC_002484.1 | + | 49386 | 0.69 | 0.664288 |
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Target: 5'- uCCUGuuuUGCgGGCGUCACugcuCGAUccucccuucaGGCCa -3' miRNA: 3'- -GGAU---GCGgCCGCAGUGuu--GUUA----------CCGG- -5' |
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| 8901 | 3' | -53.5 | NC_002484.1 | + | 50990 | 0.68 | 0.750154 |
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Target: 5'- -gUACGCggcgaCGGCGUUcuGCAAU--UGGCCa -3' miRNA: 3'- ggAUGCG-----GCCGCAG--UGUUGuuACCGG- -5' |
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| 8901 | 3' | -53.5 | NC_002484.1 | + | 51839 | 0.66 | 0.828345 |
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Target: 5'- uCC-GCGCCGGCuUgGCGuGCuuguccuuGAUGGCCa -3' miRNA: 3'- -GGaUGCGGCCGcAgUGU-UG--------UUACCGG- -5' |
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| 8901 | 3' | -53.5 | NC_002484.1 | + | 51884 | 0.71 | 0.54398 |
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Target: 5'- cCUUACGCgccuugaucuccCGGCGguugCGguCGGCGAUGGCCu -3' miRNA: 3'- -GGAUGCG------------GCCGCa---GU--GUUGUUACCGG- -5' |
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| 8901 | 3' | -53.5 | NC_002484.1 | + | 52148 | 0.69 | 0.653303 |
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Target: 5'- aCUUGCuggaGCCGGCGUUACA-----GGCCu -3' miRNA: 3'- -GGAUG----CGGCCGCAGUGUuguuaCCGG- -5' |
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| 8901 | 3' | -53.5 | NC_002484.1 | + | 53966 | 0.74 | 0.394151 |
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Target: 5'- aCUA-GCCGGCGUCACGcccgcagaGCAagGaGCCg -3' miRNA: 3'- gGAUgCGGCCGCAGUGU--------UGUuaC-CGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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