Results 21 - 40 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8956 | 5' | -59.5 | NC_002512.2 | + | 116379 | 0.67 | 0.802905 |
Target: 5'- uGCUgGC-CCUGCggucccCggGGCGGGUCGc -3' miRNA: 3'- -CGAgCGuGGGCGa-----GuaCCGCCCAGUc -5' |
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8956 | 5' | -59.5 | NC_002512.2 | + | 112881 | 0.66 | 0.872578 |
Target: 5'- uCUUGCGCCCGCccugCGU-GCGGccGUCGGc -3' miRNA: 3'- cGAGCGUGGGCGa---GUAcCGCC--CAGUC- -5' |
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8956 | 5' | -59.5 | NC_002512.2 | + | 110687 | 0.66 | 0.835556 |
Target: 5'- uGCUCGCGCagCCGCcgCAgGGCcaGGGcCAGc -3' miRNA: 3'- -CGAGCGUG--GGCGa-GUaCCG--CCCaGUC- -5' |
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8956 | 5' | -59.5 | NC_002512.2 | + | 108988 | 0.68 | 0.767128 |
Target: 5'- gGCcgUGUACCUGCUCGUcggcgccGGCGGcGUCGu -3' miRNA: 3'- -CGa-GCGUGGGCGAGUA-------CCGCC-CAGUc -5' |
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8956 | 5' | -59.5 | NC_002512.2 | + | 106770 | 0.67 | 0.811295 |
Target: 5'- gGCUCucgGCCCGCggCAcUGGUcgGGGUCGGg -3' miRNA: 3'- -CGAGcg-UGGGCGa-GU-ACCG--CCCAGUC- -5' |
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8956 | 5' | -59.5 | NC_002512.2 | + | 105408 | 0.66 | 0.835556 |
Target: 5'- cCUCGUAgCCGUugUCGUGGCGccGGcUCAGc -3' miRNA: 3'- cGAGCGUgGGCG--AGUACCGC--CC-AGUC- -5' |
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8956 | 5' | -59.5 | NC_002512.2 | + | 94336 | 0.68 | 0.749898 |
Target: 5'- cGCUCGacccagaaGCCCaGCUCGUGGCGccuGG-CGGc -3' miRNA: 3'- -CGAGCg-------UGGG-CGAGUACCGC---CCaGUC- -5' |
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8956 | 5' | -59.5 | NC_002512.2 | + | 89576 | 0.67 | 0.794375 |
Target: 5'- aGCUCGcCugCgGCggGUGGCGGGaugucgCGGg -3' miRNA: 3'- -CGAGC-GugGgCGagUACCGCCCa-----GUC- -5' |
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8956 | 5' | -59.5 | NC_002512.2 | + | 82447 | 0.69 | 0.712587 |
Target: 5'- gGCgUCGguCCCGCUCccgaaGGCGGGgaaGGg -3' miRNA: 3'- -CG-AGCguGGGCGAGua---CCGCCCag-UC- -5' |
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8956 | 5' | -59.5 | NC_002512.2 | + | 79475 | 0.68 | 0.740691 |
Target: 5'- gGCgaccgCGCGCCCGUg---GGCGGuUCGGg -3' miRNA: 3'- -CGa----GCGUGGGCGaguaCCGCCcAGUC- -5' |
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8956 | 5' | -59.5 | NC_002512.2 | + | 77741 | 0.72 | 0.547711 |
Target: 5'- cGCUCGCaaucggacACCCGCUCGcgaaGGaucccccagacucCGGGUCGGg -3' miRNA: 3'- -CGAGCG--------UGGGCGAGUa---CC-------------GCCCAGUC- -5' |
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8956 | 5' | -59.5 | NC_002512.2 | + | 74388 | 0.67 | 0.827627 |
Target: 5'- -gUCGCAgCaGCUCAUGG-GGGUcCGGa -3' miRNA: 3'- cgAGCGUgGgCGAGUACCgCCCA-GUC- -5' |
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8956 | 5' | -59.5 | NC_002512.2 | + | 74005 | 0.66 | 0.850156 |
Target: 5'- --cCGCGCCCGCcgUCggggacgaacagcGUGGCcGGGUaCAGg -3' miRNA: 3'- cgaGCGUGGGCG--AG-------------UACCG-CCCA-GUC- -5' |
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8956 | 5' | -59.5 | NC_002512.2 | + | 59684 | 0.68 | 0.776927 |
Target: 5'- cGCgaggaGCACCCGCUCAUGGCc------ -3' miRNA: 3'- -CGag---CGUGGGCGAGUACCGcccaguc -5' |
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8956 | 5' | -59.5 | NC_002512.2 | + | 54243 | 0.67 | 0.827627 |
Target: 5'- aCUCGCACguCCGCagcggCAgggcGGCGGcGUCGGc -3' miRNA: 3'- cGAGCGUG--GGCGa----GUa---CCGCC-CAGUC- -5' |
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8956 | 5' | -59.5 | NC_002512.2 | + | 52835 | 0.69 | 0.712587 |
Target: 5'- gGCUCuccacgaacaaGCACCUacgGCUCAacUGGCGGGacgcgCAGg -3' miRNA: 3'- -CGAG-----------CGUGGG---CGAGU--ACCGCCCa----GUC- -5' |
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8956 | 5' | -59.5 | NC_002512.2 | + | 45279 | 0.77 | 0.270511 |
Target: 5'- cGCUCGCGCUCGuCUC---GCGGGUCGGa -3' miRNA: 3'- -CGAGCGUGGGC-GAGuacCGCCCAGUC- -5' |
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8956 | 5' | -59.5 | NC_002512.2 | + | 22790 | 0.72 | 0.52044 |
Target: 5'- aGCUgCGCGCCCGCUaccacaagGGCGGGaUCc- -3' miRNA: 3'- -CGA-GCGUGGGCGAgua-----CCGCCC-AGuc -5' |
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8956 | 5' | -59.5 | NC_002512.2 | + | 21538 | 0.66 | 0.843318 |
Target: 5'- --aCGCACCUGCUCcucGG-GGGUgAGg -3' miRNA: 3'- cgaGCGUGGGCGAGua-CCgCCCAgUC- -5' |
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8956 | 5' | -59.5 | NC_002512.2 | + | 20868 | 0.74 | 0.414412 |
Target: 5'- gGC-CGC-CCCGCUCAUGGCGGcGacgaCGGg -3' miRNA: 3'- -CGaGCGuGGGCGAGUACCGCC-Ca---GUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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