Results 1 - 20 of 85 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8966 | 5' | -59.6 | NC_002512.2 | + | 4581 | 0.66 | 0.792184 |
Target: 5'- -gGGGCGGCggaGGagGCGgCGCCGCGg- -3' miRNA: 3'- uaCUUGCCGa--CCg-UGUgGCGGCGCaa -5' |
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8966 | 5' | -59.6 | NC_002512.2 | + | 4893 | 0.71 | 0.50315 |
Target: 5'- -gGAGCGGCgacgggaagagGGCGcCGCCGCCGCc-- -3' miRNA: 3'- uaCUUGCCGa----------CCGU-GUGGCGGCGcaa -5' |
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8966 | 5' | -59.6 | NC_002512.2 | + | 7383 | 0.66 | 0.826243 |
Target: 5'- -cGGGCGGCcaGGCGCagaaggcggccACCGCCaCGUUg -3' miRNA: 3'- uaCUUGCCGa-CCGUG-----------UGGCGGcGCAA- -5' |
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8966 | 5' | -59.6 | NC_002512.2 | + | 27974 | 0.67 | 0.765189 |
Target: 5'- gAUGGGCGGCcgGGCGagauuCCGgCGCGg- -3' miRNA: 3'- -UACUUGCCGa-CCGUgu---GGCgGCGCaa -5' |
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8966 | 5' | -59.6 | NC_002512.2 | + | 29017 | 0.68 | 0.702599 |
Target: 5'- gGUGGGCGGCgUGGUcgACGCCaugcggcagagacgcGCCGCGc- -3' miRNA: 3'- -UACUUGCCG-ACCG--UGUGG---------------CGGCGCaa -5' |
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8966 | 5' | -59.6 | NC_002512.2 | + | 31657 | 0.69 | 0.619706 |
Target: 5'- -cGGACGGCgcgGGCGCGgCCGUgGUGg- -3' miRNA: 3'- uaCUUGCCGa--CCGUGU-GGCGgCGCaa -5' |
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8966 | 5' | -59.6 | NC_002512.2 | + | 37195 | 0.66 | 0.809516 |
Target: 5'- --aGACGGCcucGGCGCGCCGCCa---- -3' miRNA: 3'- uacUUGCCGa--CCGUGUGGCGGcgcaa -5' |
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8966 | 5' | -59.6 | NC_002512.2 | + | 45423 | 0.67 | 0.765189 |
Target: 5'- --cGACGGC-GGCGC-CgGCCGCGg- -3' miRNA: 3'- uacUUGCCGaCCGUGuGgCGGCGCaa -5' |
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8966 | 5' | -59.6 | NC_002512.2 | + | 49211 | 0.68 | 0.709395 |
Target: 5'- -cGGGCGGCgugugagaucuucggGGC-CACCGCgGCGg- -3' miRNA: 3'- uaCUUGCCGa--------------CCGuGUGGCGgCGCaa -5' |
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8966 | 5' | -59.6 | NC_002512.2 | + | 52757 | 0.66 | 0.800922 |
Target: 5'- -aGAACGcgcgggcccuGCUGGCGCACCcggaccuccugGUCGCGg- -3' miRNA: 3'- uaCUUGC----------CGACCGUGUGG-----------CGGCGCaa -5' |
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8966 | 5' | -59.6 | NC_002512.2 | + | 56157 | 0.66 | 0.792184 |
Target: 5'- gAUGAugGGgUGGUugccgaGCACgGUCGCGg- -3' miRNA: 3'- -UACUugCCgACCG------UGUGgCGGCGCaa -5' |
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8966 | 5' | -59.6 | NC_002512.2 | + | 57072 | 0.7 | 0.599898 |
Target: 5'- -cGAACGGUaGGCGCACCGCaaGCu-- -3' miRNA: 3'- uaCUUGCCGaCCGUGUGGCGg-CGcaa -5' |
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8966 | 5' | -59.6 | NC_002512.2 | + | 58206 | 0.66 | 0.817959 |
Target: 5'- aGUGGgcGCGGC-GcGCACcCCGCgCGCGUa -3' miRNA: 3'- -UACU--UGCCGaC-CGUGuGGCG-GCGCAa -5' |
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8966 | 5' | -59.6 | NC_002512.2 | + | 62455 | 0.69 | 0.619706 |
Target: 5'- -cGAACGcGCaGGCGUGCCGCCGCa-- -3' miRNA: 3'- uaCUUGC-CGaCCGUGUGGCGGCGcaa -5' |
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8966 | 5' | -59.6 | NC_002512.2 | + | 68904 | 0.7 | 0.550878 |
Target: 5'- -gGGGCGGUcgUGcuGCugGCCGCCGCGg- -3' miRNA: 3'- uaCUUGCCG--AC--CGugUGGCGGCGCaa -5' |
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8966 | 5' | -59.6 | NC_002512.2 | + | 69083 | 0.78 | 0.206623 |
Target: 5'- -gGGGCGGCUGGUACuGCUGCCGUGg- -3' miRNA: 3'- uaCUUGCCGACCGUG-UGGCGGCGCaa -5' |
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8966 | 5' | -59.6 | NC_002512.2 | + | 69874 | 0.66 | 0.800922 |
Target: 5'- -cGAACGGCccUGGUucgGCACCGCCa---- -3' miRNA: 3'- uaCUUGCCG--ACCG---UGUGGCGGcgcaa -5' |
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8966 | 5' | -59.6 | NC_002512.2 | + | 71011 | 0.66 | 0.783311 |
Target: 5'- -aGGACGGggGGC-CGCCGCCGg--- -3' miRNA: 3'- uaCUUGCCgaCCGuGUGGCGGCgcaa -5' |
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8966 | 5' | -59.6 | NC_002512.2 | + | 71379 | 0.66 | 0.792184 |
Target: 5'- -gGAGC-GCUGGCGCAg-GCCGCGg- -3' miRNA: 3'- uaCUUGcCGACCGUGUggCGGCGCaa -5' |
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8966 | 5' | -59.6 | NC_002512.2 | + | 74618 | 0.66 | 0.817959 |
Target: 5'- -gGAGCGGa--GCGCgagACCGCCGCGc- -3' miRNA: 3'- uaCUUGCCgacCGUG---UGGCGGCGCaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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