Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8975 | 5' | -56 | NC_002512.2 | + | 59404 | 0.66 | 0.932565 |
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Target: 5'- gGCgCUGGAGAucGAcacgcgcugcaaccUGUGCGCCAUcGUg -3' miRNA: 3'- -CGgGACCUCUu-CU--------------ACGCGCGGUA-CAg -5' |
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| 8975 | 5' | -56 | NC_002512.2 | + | 72028 | 0.67 | 0.923799 |
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Target: 5'- cGCCCUGGAuGGGcagcuUGCGCuGCgCGUGcCg -3' miRNA: 3'- -CGGGACCUcUUCu----ACGCG-CG-GUACaG- -5' |
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| 8975 | 5' | -56 | NC_002512.2 | + | 60845 | 0.67 | 0.918333 |
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Target: 5'- cGCCC--GAGGAGAUGCcgGCGCCGc--- -3' miRNA: 3'- -CGGGacCUCUUCUACG--CGCGGUacag -5' |
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| 8975 | 5' | -56 | NC_002512.2 | + | 32177 | 0.67 | 0.912637 |
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Target: 5'- aCCCUGGGGuc---GCGgGCCAUGg- -3' miRNA: 3'- cGGGACCUCuucuaCGCgCGGUACag -5' |
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| 8975 | 5' | -56 | NC_002512.2 | + | 121525 | 0.68 | 0.89419 |
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Target: 5'- aUCCUcGGGGAGcuccgGCGCGCCGggGUCg -3' miRNA: 3'- cGGGA-CCUCUUcua--CGCGCGGUa-CAG- -5' |
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| 8975 | 5' | -56 | NC_002512.2 | + | 91909 | 0.73 | 0.628713 |
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Target: 5'- cUCCUGGGGcGGcgGCGCGCC--GUCg -3' miRNA: 3'- cGGGACCUCuUCuaCGCGCGGuaCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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