Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8975 | 5' | -56 | NC_002512.2 | + | 220529 | 0.7 | 0.819099 |
Target: 5'- -aCCUGGAGGAGcUGCuGCGCgAgggcGUCa -3' miRNA: 3'- cgGGACCUCUUCuACG-CGCGgUa---CAG- -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 222695 | 0.7 | 0.793083 |
Target: 5'- cGCCCggcacGGGGAGGAgUGCGuCGCCAccUGcCu -3' miRNA: 3'- -CGGGa----CCUCUUCU-ACGC-GCGGU--ACaG- -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 92167 | 0.7 | 0.784121 |
Target: 5'- uCCCUGGAGAA----CGCcgGCCAUGUCu -3' miRNA: 3'- cGGGACCUCUUcuacGCG--CGGUACAG- -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 127600 | 0.72 | 0.708419 |
Target: 5'- cGCCCUGGcGGucGAcGCGCGCCGa--- -3' miRNA: 3'- -CGGGACC-UCuuCUaCGCGCGGUacag -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 91909 | 0.73 | 0.628713 |
Target: 5'- cUCCUGGGGcGGcgGCGCGCC--GUCg -3' miRNA: 3'- cGGGACCUCuUCuaCGCGCGGuaCAG- -5' |
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8975 | 5' | -56 | NC_002512.2 | + | 197739 | 1.1 | 0.003394 |
Target: 5'- gGCCCUGGAGAAGAUGCGCGCCAUGUCc -3' miRNA: 3'- -CGGGACCUCUUCUACGCGCGGUACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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