Results 1 - 20 of 94 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8998 | 5' | -53.6 | NC_002512.2 | + | 212392 | 0.66 | 0.993511 |
Target: 5'- uCGAGAUCgaggaggcggccgaCGAGGACGACgg--GCGg -3' miRNA: 3'- -GCUCUGGag------------GCUCCUGCUGaagaUGC- -5' |
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8998 | 5' | -53.6 | NC_002512.2 | + | 134651 | 0.66 | 0.993422 |
Target: 5'- -cGGAcgcccCCUCCgGAGaACGAUUUCUACGa -3' miRNA: 3'- gcUCU-----GGAGG-CUCcUGCUGAAGAUGC- -5' |
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8998 | 5' | -53.6 | NC_002512.2 | + | 100069 | 0.66 | 0.993422 |
Target: 5'- gGGGGCCgcgggCCGGGGACGGa----GCGg -3' miRNA: 3'- gCUCUGGa----GGCUCCUGCUgaagaUGC- -5' |
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8998 | 5' | -53.6 | NC_002512.2 | + | 150318 | 0.66 | 0.993422 |
Target: 5'- gCGAGACCggcggCGAGGACGcCgcg-GCGg -3' miRNA: 3'- -GCUCUGGag---GCUCCUGCuGaagaUGC- -5' |
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8998 | 5' | -53.6 | NC_002512.2 | + | 167165 | 0.66 | 0.993422 |
Target: 5'- gCGAGGCCgUCGAccGCGACgcgCUGCGc -3' miRNA: 3'- -GCUCUGGaGGCUccUGCUGaa-GAUGC- -5' |
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8998 | 5' | -53.6 | NC_002512.2 | + | 200255 | 0.66 | 0.993422 |
Target: 5'- gGGGACga-CGAGGACGACg---ACGg -3' miRNA: 3'- gCUCUGgagGCUCCUGCUGaagaUGC- -5' |
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8998 | 5' | -53.6 | NC_002512.2 | + | 103893 | 0.66 | 0.993332 |
Target: 5'- aCGAGACCUUCacGGcccugaaGCG-CUUCUGCGu -3' miRNA: 3'- -GCUCUGGAGGcuCC-------UGCuGAAGAUGC- -5' |
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8998 | 5' | -53.6 | NC_002512.2 | + | 11741 | 0.66 | 0.992576 |
Target: 5'- aCGAGGCCgggagcgacgacgaCGAcGACGugUUCUACc -3' miRNA: 3'- -GCUCUGGag------------GCUcCUGCugAAGAUGc -5' |
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8998 | 5' | -53.6 | NC_002512.2 | + | 142260 | 0.66 | 0.992477 |
Target: 5'- uGGGAuccggucgucguCCgggCCGGGGACGGCgUCgACGg -3' miRNA: 3'- gCUCU------------GGa--GGCUCCUGCUGaAGaUGC- -5' |
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8998 | 5' | -53.6 | NC_002512.2 | + | 123174 | 0.66 | 0.992477 |
Target: 5'- cCGAGGCCgaggCCGAGGcGgGACggg-ACGa -3' miRNA: 3'- -GCUCUGGa---GGCUCC-UgCUGaagaUGC- -5' |
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8998 | 5' | -53.6 | NC_002512.2 | + | 159960 | 0.66 | 0.992477 |
Target: 5'- gGAGACCUaCCGGGaGAccuCGACcugCUACc -3' miRNA: 3'- gCUCUGGA-GGCUC-CU---GCUGaa-GAUGc -5' |
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8998 | 5' | -53.6 | NC_002512.2 | + | 94388 | 0.66 | 0.992477 |
Target: 5'- gCGuGGCCaugaUCuUGAGGACGGCggUCUugGc -3' miRNA: 3'- -GCuCUGG----AG-GCUCCUGCUGa-AGAugC- -5' |
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8998 | 5' | -53.6 | NC_002512.2 | + | 162901 | 0.66 | 0.992477 |
Target: 5'- uGAGcCCUUCGAGGAgGACaccgccaacUUCcGCGu -3' miRNA: 3'- gCUCuGGAGGCUCCUgCUG---------AAGaUGC- -5' |
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8998 | 5' | -53.6 | NC_002512.2 | + | 108370 | 0.66 | 0.992477 |
Target: 5'- gGAGcuCC-CCGAGGAUGACgUUCaUGCu -3' miRNA: 3'- gCUCu-GGaGGCUCCUGCUG-AAG-AUGc -5' |
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8998 | 5' | -53.6 | NC_002512.2 | + | 143369 | 0.66 | 0.992477 |
Target: 5'- uGGGGCCUCCccgcucaAGGACGGCUauaaaccgUCUccucACGu -3' miRNA: 3'- gCUCUGGAGGc------UCCUGCUGA--------AGA----UGC- -5' |
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8998 | 5' | -53.6 | NC_002512.2 | + | 12384 | 0.66 | 0.991426 |
Target: 5'- cCGAGcucGCCUUCGGGcGGCGGCg---GCGg -3' miRNA: 3'- -GCUC---UGGAGGCUC-CUGCUGaagaUGC- -5' |
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8998 | 5' | -53.6 | NC_002512.2 | + | 209062 | 0.66 | 0.991426 |
Target: 5'- aCGGcuCCUCCGAcGACGuCUUCgUGCGg -3' miRNA: 3'- -GCUcuGGAGGCUcCUGCuGAAG-AUGC- -5' |
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8998 | 5' | -53.6 | NC_002512.2 | + | 89327 | 0.66 | 0.991426 |
Target: 5'- uGAcGACCUUCGAGGuagaggaccagaACGGCgUCgagGCGg -3' miRNA: 3'- gCU-CUGGAGGCUCC------------UGCUGaAGa--UGC- -5' |
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8998 | 5' | -53.6 | NC_002512.2 | + | 13237 | 0.66 | 0.991426 |
Target: 5'- --cGugCUgCG-GGACGACUUCcGCGu -3' miRNA: 3'- gcuCugGAgGCuCCUGCUGAAGaUGC- -5' |
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8998 | 5' | -53.6 | NC_002512.2 | + | 47891 | 0.66 | 0.990263 |
Target: 5'- --uGACC-CCGAGGACGcCgccacCUACGu -3' miRNA: 3'- gcuCUGGaGGCUCCUGCuGaa---GAUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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