Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9176 | 3' | -58.6 | NC_002512.2 | + | 123137 | 0.66 | 0.908618 |
Target: 5'- aGCCGgacGCGGAGCGccGCCGGGg---- -3' miRNA: 3'- -CGGCac-CGCCUCGCa-CGGUCUguagc -5' |
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9176 | 3' | -58.6 | NC_002512.2 | + | 121357 | 0.66 | 0.908618 |
Target: 5'- gGCCGUuggacGGCGGAGaggGCCGGAgAggagggCGa -3' miRNA: 3'- -CGGCA-----CCGCCUCgcaCGGUCUgUa-----GC- -5' |
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9176 | 3' | -58.6 | NC_002512.2 | + | 153422 | 0.66 | 0.883786 |
Target: 5'- --gGUGGCGGAaugGCGgcaggccgGCCGGGCG-CGg -3' miRNA: 3'- cggCACCGCCU---CGCa-------CGGUCUGUaGC- -5' |
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9176 | 3' | -58.6 | NC_002512.2 | + | 225730 | 0.66 | 0.889663 |
Target: 5'- aGCCGcGGCgacucgcucagucGGGGCGcgGCCgGGACAUgGg -3' miRNA: 3'- -CGGCaCCG-------------CCUCGCa-CGG-UCUGUAgC- -5' |
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9176 | 3' | -58.6 | NC_002512.2 | + | 195785 | 0.66 | 0.890305 |
Target: 5'- aGCC-UGGaCGuGGGCGUGUUgcuccgcugcggGGACAUCGu -3' miRNA: 3'- -CGGcACC-GC-CUCGCACGG------------UCUGUAGC- -5' |
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9176 | 3' | -58.6 | NC_002512.2 | + | 158985 | 0.66 | 0.896619 |
Target: 5'- gGCCacGGCGGGGCGgaggcggcgGCCccGGCGUCc -3' miRNA: 3'- -CGGcaCCGCCUCGCa--------CGGu-CUGUAGc -5' |
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9176 | 3' | -58.6 | NC_002512.2 | + | 75546 | 0.67 | 0.870146 |
Target: 5'- cGCCGUGGCGGAggacgacgacGCGgacgacuCCGGAUccCGg -3' miRNA: 3'- -CGGCACCGCCU----------CGCac-----GGUCUGuaGC- -5' |
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9176 | 3' | -58.6 | NC_002512.2 | + | 139983 | 0.67 | 0.863034 |
Target: 5'- cCCGUGcCGGA-CGUcCCAGACAUCu -3' miRNA: 3'- cGGCACcGCCUcGCAcGGUCUGUAGc -5' |
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9176 | 3' | -58.6 | NC_002512.2 | + | 228223 | 0.67 | 0.855734 |
Target: 5'- gGCCGggcgagacGGCGGgaagGGCGaacgUGCCGGACG-CGg -3' miRNA: 3'- -CGGCa-------CCGCC----UCGC----ACGGUCUGUaGC- -5' |
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9176 | 3' | -58.6 | NC_002512.2 | + | 224144 | 0.67 | 0.84825 |
Target: 5'- cGCCGggaGGCGGAcGCGUgagGCgGGGCGggggCGc -3' miRNA: 3'- -CGGCa--CCGCCU-CGCA---CGgUCUGUa---GC- -5' |
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9176 | 3' | -58.6 | NC_002512.2 | + | 222250 | 0.67 | 0.84597 |
Target: 5'- cGCCGUGGCGGGucucgugauggccuGCGcccUGCuCGGGCGc-- -3' miRNA: 3'- -CGGCACCGCCU--------------CGC---ACG-GUCUGUagc -5' |
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9176 | 3' | -58.6 | NC_002512.2 | + | 134538 | 0.67 | 0.840588 |
Target: 5'- cGCUGUGGCGacaGAGCGaGCUGGAgcugAUCGg -3' miRNA: 3'- -CGGCACCGC---CUCGCaCGGUCUg---UAGC- -5' |
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9176 | 3' | -58.6 | NC_002512.2 | + | 177679 | 0.67 | 0.835909 |
Target: 5'- cGCCGccaguuugcgguagaUGGggaucaGGGGCuucccccaggucGUGCCGGACAUCGu -3' miRNA: 3'- -CGGC---------------ACCg-----CCUCG------------CACGGUCUGUAGC- -5' |
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9176 | 3' | -58.6 | NC_002512.2 | + | 127784 | 0.67 | 0.831963 |
Target: 5'- gGCCGaGGCcgcacagGGAGaCGUGCCGGAagaacUCGg -3' miRNA: 3'- -CGGCaCCG-------CCUC-GCACGGUCUgu---AGC- -5' |
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9176 | 3' | -58.6 | NC_002512.2 | + | 92543 | 0.68 | 0.78253 |
Target: 5'- gGCCGcGGCGGccGCG-GCgAcGACGUCGg -3' miRNA: 3'- -CGGCaCCGCCu-CGCaCGgU-CUGUAGC- -5' |
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9176 | 3' | -58.6 | NC_002512.2 | + | 193452 | 0.68 | 0.79125 |
Target: 5'- cGCCgGUGGCgGGAGCGgGCCcuccgGGACGa-- -3' miRNA: 3'- -CGG-CACCG-CCUCGCaCGG-----UCUGUagc -5' |
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9176 | 3' | -58.6 | NC_002512.2 | + | 217793 | 0.68 | 0.79125 |
Target: 5'- gGCCGgcUGGCGGAucgGCGUcGCCGucccGAuCGUCGa -3' miRNA: 3'- -CGGC--ACCGCCU---CGCA-CGGU----CU-GUAGC- -5' |
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9176 | 3' | -58.6 | NC_002512.2 | + | 143684 | 0.68 | 0.79984 |
Target: 5'- aGCCG-GGCGGGGU--GUCGGGCA-CGg -3' miRNA: 3'- -CGGCaCCGCCUCGcaCGGUCUGUaGC- -5' |
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9176 | 3' | -58.6 | NC_002512.2 | + | 185998 | 0.68 | 0.823949 |
Target: 5'- cGCCGUGGUGGGuCGU-CCGGcaccuggacgccuGCGUCGc -3' miRNA: 3'- -CGGCACCGCCUcGCAcGGUC-------------UGUAGC- -5' |
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9176 | 3' | -58.6 | NC_002512.2 | + | 226844 | 0.68 | 0.79125 |
Target: 5'- cGCCGUcaaGGCGGAGCaGgccGCCAGGgAggccCGg -3' miRNA: 3'- -CGGCA---CCGCCUCG-Ca--CGGUCUgUa---GC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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