Results 1 - 20 of 49 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9243 | 3' | -60.4 | NC_002512.2 | + | 116236 | 0.66 | 0.84561 |
Target: 5'- --cCCCCGGAGAaccacgaggccaCCCUgcaGGugCUGcGGa -3' miRNA: 3'- gcaGGGGCCUCU------------GGGA---CCugGACaCC- -5' |
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9243 | 3' | -60.4 | NC_002512.2 | + | 118112 | 0.66 | 0.838058 |
Target: 5'- gCGUCCCagCGGGGcuCCCUGGGCgacGUGa -3' miRNA: 3'- -GCAGGG--GCCUCu-GGGACCUGga-CACc -5' |
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9243 | 3' | -60.4 | NC_002512.2 | + | 164021 | 0.66 | 0.833448 |
Target: 5'- aGcCCCUGGAGAacaccgacuuucagaUCCUGGAgCUGUa- -3' miRNA: 3'- gCaGGGGCCUCU---------------GGGACCUgGACAcc -5' |
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9243 | 3' | -60.4 | NC_002512.2 | + | 205096 | 0.66 | 0.830343 |
Target: 5'- cCGUCCUCGGGGggcgcGCCC-GGACCcGagcgGGu -3' miRNA: 3'- -GCAGGGGCCUC-----UGGGaCCUGGaCa---CC- -5' |
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9243 | 3' | -60.4 | NC_002512.2 | + | 106978 | 0.66 | 0.830343 |
Target: 5'- uCGUCCCCGuagaaggccGGGACgCCcGGGCCg--GGg -3' miRNA: 3'- -GCAGGGGC---------CUCUG-GGaCCUGGacaCC- -5' |
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9243 | 3' | -60.4 | NC_002512.2 | + | 222377 | 0.66 | 0.852994 |
Target: 5'- -uUCCCCGGcGGGCUCUGGcuCCUcaUGGa -3' miRNA: 3'- gcAGGGGCC-UCUGGGACCu-GGAc-ACC- -5' |
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9243 | 3' | -60.4 | NC_002512.2 | + | 105084 | 0.66 | 0.860205 |
Target: 5'- aGUCuCCCGGcuccgucagcuGGACCC-GGACCcG-GGa -3' miRNA: 3'- gCAG-GGGCC-----------UCUGGGaCCUGGaCaCC- -5' |
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9243 | 3' | -60.4 | NC_002512.2 | + | 60989 | 0.66 | 0.84561 |
Target: 5'- aCGUCuUCCGcGAGcACCC-GGACCUGg-- -3' miRNA: 3'- -GCAG-GGGC-CUC-UGGGaCCUGGACacc -5' |
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9243 | 3' | -60.4 | NC_002512.2 | + | 80883 | 0.66 | 0.830343 |
Target: 5'- gGUCCgCGGcGGCCCcccGGCCgGUGGu -3' miRNA: 3'- gCAGGgGCCuCUGGGac-CUGGaCACC- -5' |
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9243 | 3' | -60.4 | NC_002512.2 | + | 61450 | 0.67 | 0.789557 |
Target: 5'- -aUCCugCUGGuGGCCCgGGACCUGUaccGGg -3' miRNA: 3'- gcAGG--GGCCuCUGGGaCCUGGACA---CC- -5' |
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9243 | 3' | -60.4 | NC_002512.2 | + | 126709 | 0.67 | 0.822473 |
Target: 5'- --aCCCCGGGGGUCCUGGAUCa---- -3' miRNA: 3'- gcaGGGGCCUCUGGGACCUGGacacc -5' |
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9243 | 3' | -60.4 | NC_002512.2 | + | 134199 | 0.67 | 0.797987 |
Target: 5'- cCGUCCCCgccGGAGGCCCggcGGACg----- -3' miRNA: 3'- -GCAGGGG---CCUCUGGGa--CCUGgacacc -5' |
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9243 | 3' | -60.4 | NC_002512.2 | + | 55629 | 0.67 | 0.814452 |
Target: 5'- --aCCCCGG-GACCC-GGACCcG-GGc -3' miRNA: 3'- gcaGGGGCCuCUGGGaCCUGGaCaCC- -5' |
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9243 | 3' | -60.4 | NC_002512.2 | + | 11436 | 0.67 | 0.822473 |
Target: 5'- gGUCCgCGGGGACgCCgcggacggGGGCCgg-GGa -3' miRNA: 3'- gCAGGgGCCUCUG-GGa-------CCUGGacaCC- -5' |
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9243 | 3' | -60.4 | NC_002512.2 | + | 101323 | 0.67 | 0.781004 |
Target: 5'- gGcCCCCGGAGGCCgucgaaggUGGACaUGUGa -3' miRNA: 3'- gCaGGGGCCUCUGGg-------ACCUGgACACc -5' |
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9243 | 3' | -60.4 | NC_002512.2 | + | 113506 | 0.67 | 0.781004 |
Target: 5'- cCG-CCCCGGGGACCgCaGGGCCa---- -3' miRNA: 3'- -GCaGGGGCCUCUGG-GaCCUGGacacc -5' |
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9243 | 3' | -60.4 | NC_002512.2 | + | 192985 | 0.67 | 0.797987 |
Target: 5'- gGUCCuCCGGcGcGCUCUGGACCgcgGGc -3' miRNA: 3'- gCAGG-GGCCuC-UGGGACCUGGacaCC- -5' |
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9243 | 3' | -60.4 | NC_002512.2 | + | 37021 | 0.68 | 0.727511 |
Target: 5'- gCGUCCCCGGGGACCaCUGucACUcG-GGu -3' miRNA: 3'- -GCAGGGGCCUCUGG-GACc-UGGaCaCC- -5' |
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9243 | 3' | -60.4 | NC_002512.2 | + | 100807 | 0.68 | 0.727511 |
Target: 5'- cCGUCCUCGGGGucgaCCUGGACgaGg-- -3' miRNA: 3'- -GCAGGGGCCUCug--GGACCUGgaCacc -5' |
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9243 | 3' | -60.4 | NC_002512.2 | + | 159940 | 0.68 | 0.745707 |
Target: 5'- aCGUCCCCGGuGGCC--GGACCg---- -3' miRNA: 3'- -GCAGGGGCCuCUGGgaCCUGGacacc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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