Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9256 | 5' | -46.5 | NC_002512.2 | + | 212098 | 0.66 | 0.999998 |
Target: 5'- aGCGCGUgCUCGCCGa------GUUCCa -3' miRNA: 3'- cUGUGCA-GAGCGGCaagauuaUAAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 221187 | 0.66 | 0.999999 |
Target: 5'- aGAgGCGUC-CGCUGUUUU-GUGcUCCc -3' miRNA: 3'- -CUgUGCAGaGCGGCAAGAuUAUaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 83288 | 0.66 | 0.999999 |
Target: 5'- aGAgGCGUCUggggcCGCCGUUCgucucgguUGAUAUacuUCUg -3' miRNA: 3'- -CUgUGCAGA-----GCGGCAAG--------AUUAUA---AGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 186143 | 0.66 | 0.999998 |
Target: 5'- cGAUACGga-CGCCGUUCg-----UCCg -3' miRNA: 3'- -CUGUGCagaGCGGCAAGauuauaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 21909 | 0.66 | 0.999998 |
Target: 5'- aGCACGUCUCGUCGccgCUAcgGa--- -3' miRNA: 3'- cUGUGCAGAGCGGCaa-GAUuaUaagg -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 66499 | 0.66 | 0.999997 |
Target: 5'- cGugACGuUUUCGCCcg--UGAUGUUCCa -3' miRNA: 3'- -CugUGC-AGAGCGGcaagAUUAUAAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 13653 | 0.66 | 0.999995 |
Target: 5'- cGAC-CGUCUCGCCGcggCUccagcUCCc -3' miRNA: 3'- -CUGuGCAGAGCGGCaa-GAuuauaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 17309 | 0.66 | 0.999997 |
Target: 5'- -uCGCGUCgUCGCCGccgUCgucGUcgUCCc -3' miRNA: 3'- cuGUGCAG-AGCGGCa--AGau-UAuaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 128120 | 0.66 | 0.999999 |
Target: 5'- cGGCGcCGUCUCGCCGaaggucaggcggcggCUGGUcUUCg -3' miRNA: 3'- -CUGU-GCAGAGCGGCaa-------------GAUUAuAAGg -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 38237 | 0.66 | 0.999998 |
Target: 5'- --gACGUCUcCGCCGUcCUGGc--UCCg -3' miRNA: 3'- cugUGCAGA-GCGGCAaGAUUauaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 178761 | 0.66 | 0.999999 |
Target: 5'- -gUACGUCgCGUCGUUCUcGAUGUacUUCg -3' miRNA: 3'- cuGUGCAGaGCGGCAAGA-UUAUA--AGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 181757 | 0.67 | 0.99998 |
Target: 5'- uACAUGUCUggucagauucuguaUGuCCGUUCUGAgg-UCCg -3' miRNA: 3'- cUGUGCAGA--------------GC-GGCAAGAUUauaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 76516 | 0.67 | 0.99999 |
Target: 5'- cGCACGUCcagUUGCCGg-CUGAcGUUCUg -3' miRNA: 3'- cUGUGCAG---AGCGGCaaGAUUaUAAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 176688 | 0.67 | 0.99999 |
Target: 5'- uGGCGCG--UCGCC-UUgUGGUGUUCCc -3' miRNA: 3'- -CUGUGCagAGCGGcAAgAUUAUAAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 5497 | 0.67 | 0.999975 |
Target: 5'- cGCGCGUCcgCGCCGgggUCgcgaggUCCc -3' miRNA: 3'- cUGUGCAGa-GCGGCa--AGauuauaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 149931 | 0.67 | 0.999975 |
Target: 5'- -cCGCGUCaUCGCCGcgCUuuccUUCCu -3' miRNA: 3'- cuGUGCAG-AGCGGCaaGAuuauAAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 139166 | 0.68 | 0.999939 |
Target: 5'- aGGC-CGUCUUGCCGcacUCUAGgg--CCa -3' miRNA: 3'- -CUGuGCAGAGCGGCa--AGAUUauaaGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 210194 | 0.68 | 0.999939 |
Target: 5'- --uGCGUCcCGaCCG-UCUGAUcgUCCg -3' miRNA: 3'- cugUGCAGaGC-GGCaAGAUUAuaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 113170 | 0.68 | 0.999939 |
Target: 5'- -cCACGUCggacgUGCCGgggCUGcguUGUUCCa -3' miRNA: 3'- cuGUGCAGa----GCGGCaa-GAUu--AUAAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 84319 | 0.68 | 0.999939 |
Target: 5'- cGGCGCGUCgcgCGCCGcagcgUCgccgcgUCCu -3' miRNA: 3'- -CUGUGCAGa--GCGGCa----AGauuauaAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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