Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9256 | 5' | -46.5 | NC_002512.2 | + | 58760 | 1.15 | 0.01945 |
Target: 5'- gGACACGUCUCGCCGUUCUAAUAUUCCa -3' miRNA: 3'- -CUGUGCAGAGCGGCAAGAUUAUAAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 88946 | 0.76 | 0.968026 |
Target: 5'- cGGCGCGUCUCGuCCGUaucuggcucugcgUCUGGgucUCCg -3' miRNA: 3'- -CUGUGCAGAGC-GGCA-------------AGAUUauaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 144055 | 0.76 | 0.971371 |
Target: 5'- cGACcCGUCaUCGCCGUUCcuuucUGUUCCc -3' miRNA: 3'- -CUGuGCAG-AGCGGCAAGauu--AUAAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 156725 | 0.75 | 0.981424 |
Target: 5'- aGCugGUCUCGuCCGUcgUCUGGUAcUUCa -3' miRNA: 3'- cUGugCAGAGC-GGCA--AGAUUAUaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 142433 | 0.74 | 0.988565 |
Target: 5'- aGGCGCGUCUCcgguuuCCGUUCUGugcUGUUCg -3' miRNA: 3'- -CUGUGCAGAGc-----GGCAAGAUu--AUAAGg -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 222445 | 0.71 | 0.998488 |
Target: 5'- --aGCGUCgcgugCGCCGUUCUcGUcgUUCg -3' miRNA: 3'- cugUGCAGa----GCGGCAAGAuUAuaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 176880 | 0.7 | 0.999157 |
Target: 5'- uGGCugGUgUUGUCGUg--GAUGUUCCc -3' miRNA: 3'- -CUGugCAgAGCGGCAagaUUAUAAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 137837 | 0.7 | 0.999157 |
Target: 5'- aGCACGUCUCuCUGUUCUuaaauagGUUCUu -3' miRNA: 3'- cUGUGCAGAGcGGCAAGAuua----UAAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 73980 | 0.69 | 0.999896 |
Target: 5'- gGACGCGUCggCGCCGgaCUcg---UCCc -3' miRNA: 3'- -CUGUGCAGa-GCGGCaaGAuuauaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 83396 | 0.68 | 0.999939 |
Target: 5'- cGCGCGUCccCGCCGUgggGGU-UUCCg -3' miRNA: 3'- cUGUGCAGa-GCGGCAagaUUAuAAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 113170 | 0.68 | 0.999939 |
Target: 5'- -cCACGUCggacgUGCCGgggCUGcguUGUUCCa -3' miRNA: 3'- cuGUGCAGa----GCGGCaa-GAUu--AUAAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 139166 | 0.68 | 0.999939 |
Target: 5'- aGGC-CGUCUUGCCGcacUCUAGgg--CCa -3' miRNA: 3'- -CUGuGCAGAGCGGCa--AGAUUauaaGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 210194 | 0.68 | 0.999939 |
Target: 5'- --uGCGUCcCGaCCG-UCUGAUcgUCCg -3' miRNA: 3'- cugUGCAGaGC-GGCaAGAUUAuaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 84319 | 0.68 | 0.999939 |
Target: 5'- cGGCGCGUCgcgCGCCGcagcgUCgccgcgUCCu -3' miRNA: 3'- -CUGUGCAGa--GCGGCa----AGauuauaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 142307 | 0.68 | 0.999954 |
Target: 5'- cGACGCGUCcgUCGCCGU-CUGcgGa--- -3' miRNA: 3'- -CUGUGCAG--AGCGGCAaGAUuaUaagg -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 5497 | 0.67 | 0.999975 |
Target: 5'- cGCGCGUCcgCGCCGgggUCgcgaggUCCc -3' miRNA: 3'- cUGUGCAGa-GCGGCa--AGauuauaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 149931 | 0.67 | 0.999975 |
Target: 5'- -cCGCGUCaUCGCCGcgCUuuccUUCCu -3' miRNA: 3'- cuGUGCAG-AGCGGCaaGAuuauAAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 181757 | 0.67 | 0.99998 |
Target: 5'- uACAUGUCUggucagauucuguaUGuCCGUUCUGAgg-UCCg -3' miRNA: 3'- cUGUGCAGA--------------GC-GGCAAGAUUauaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 76516 | 0.67 | 0.99999 |
Target: 5'- cGCACGUCcagUUGCCGg-CUGAcGUUCUg -3' miRNA: 3'- cUGUGCAG---AGCGGCaaGAUUaUAAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 176688 | 0.67 | 0.99999 |
Target: 5'- uGGCGCG--UCGCC-UUgUGGUGUUCCc -3' miRNA: 3'- -CUGUGCagAGCGGcAAgAUUAUAAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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