Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9256 | 5' | -46.5 | NC_002512.2 | + | 5497 | 0.67 | 0.999975 |
Target: 5'- cGCGCGUCcgCGCCGgggUCgcgaggUCCc -3' miRNA: 3'- cUGUGCAGa-GCGGCa--AGauuauaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 13653 | 0.66 | 0.999995 |
Target: 5'- cGAC-CGUCUCGCCGcggCUccagcUCCc -3' miRNA: 3'- -CUGuGCAGAGCGGCaa-GAuuauaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 17309 | 0.66 | 0.999997 |
Target: 5'- -uCGCGUCgUCGCCGccgUCgucGUcgUCCc -3' miRNA: 3'- cuGUGCAG-AGCGGCa--AGau-UAuaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 21909 | 0.66 | 0.999998 |
Target: 5'- aGCACGUCUCGUCGccgCUAcgGa--- -3' miRNA: 3'- cUGUGCAGAGCGGCaa-GAUuaUaagg -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 38237 | 0.66 | 0.999998 |
Target: 5'- --gACGUCUcCGCCGUcCUGGc--UCCg -3' miRNA: 3'- cugUGCAGA-GCGGCAaGAUUauaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 58760 | 1.15 | 0.01945 |
Target: 5'- gGACACGUCUCGCCGUUCUAAUAUUCCa -3' miRNA: 3'- -CUGUGCAGAGCGGCAAGAUUAUAAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 66499 | 0.66 | 0.999997 |
Target: 5'- cGugACGuUUUCGCCcg--UGAUGUUCCa -3' miRNA: 3'- -CugUGC-AGAGCGGcaagAUUAUAAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 73980 | 0.69 | 0.999896 |
Target: 5'- gGACGCGUCggCGCCGgaCUcg---UCCc -3' miRNA: 3'- -CUGUGCAGa-GCGGCaaGAuuauaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 76516 | 0.67 | 0.99999 |
Target: 5'- cGCACGUCcagUUGCCGg-CUGAcGUUCUg -3' miRNA: 3'- cUGUGCAG---AGCGGCaaGAUUaUAAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 83288 | 0.66 | 0.999999 |
Target: 5'- aGAgGCGUCUggggcCGCCGUUCgucucgguUGAUAUacuUCUg -3' miRNA: 3'- -CUgUGCAGA-----GCGGCAAG--------AUUAUA---AGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 83396 | 0.68 | 0.999939 |
Target: 5'- cGCGCGUCccCGCCGUgggGGU-UUCCg -3' miRNA: 3'- cUGUGCAGa-GCGGCAagaUUAuAAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 84319 | 0.68 | 0.999939 |
Target: 5'- cGGCGCGUCgcgCGCCGcagcgUCgccgcgUCCu -3' miRNA: 3'- -CUGUGCAGa--GCGGCa----AGauuauaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 88946 | 0.76 | 0.968026 |
Target: 5'- cGGCGCGUCUCGuCCGUaucuggcucugcgUCUGGgucUCCg -3' miRNA: 3'- -CUGUGCAGAGC-GGCA-------------AGAUUauaAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 113170 | 0.68 | 0.999939 |
Target: 5'- -cCACGUCggacgUGCCGgggCUGcguUGUUCCa -3' miRNA: 3'- cuGUGCAGa----GCGGCaa-GAUu--AUAAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 128120 | 0.66 | 0.999999 |
Target: 5'- cGGCGcCGUCUCGCCGaaggucaggcggcggCUGGUcUUCg -3' miRNA: 3'- -CUGU-GCAGAGCGGCaa-------------GAUUAuAAGg -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 137837 | 0.7 | 0.999157 |
Target: 5'- aGCACGUCUCuCUGUUCUuaaauagGUUCUu -3' miRNA: 3'- cUGUGCAGAGcGGCAAGAuua----UAAGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 139166 | 0.68 | 0.999939 |
Target: 5'- aGGC-CGUCUUGCCGcacUCUAGgg--CCa -3' miRNA: 3'- -CUGuGCAGAGCGGCa--AGAUUauaaGG- -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 142307 | 0.68 | 0.999954 |
Target: 5'- cGACGCGUCcgUCGCCGU-CUGcgGa--- -3' miRNA: 3'- -CUGUGCAG--AGCGGCAaGAUuaUaagg -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 142433 | 0.74 | 0.988565 |
Target: 5'- aGGCGCGUCUCcgguuuCCGUUCUGugcUGUUCg -3' miRNA: 3'- -CUGUGCAGAGc-----GGCAAGAUu--AUAAGg -5' |
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9256 | 5' | -46.5 | NC_002512.2 | + | 144055 | 0.76 | 0.971371 |
Target: 5'- cGACcCGUCaUCGCCGUUCcuuucUGUUCCc -3' miRNA: 3'- -CUGuGCAG-AGCGGCAAGauu--AUAAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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