Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9353 | 3' | -48 | NC_002512.2 | + | 27024 | 0.68 | 0.999776 |
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Target: 5'- uGGUCUAUAaacGGAUCcaCCGCuGUCGGu -3' miRNA: 3'- cCCAGAUAUa--UUUAGa-GGCG-CAGCCc -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 196400 | 0.68 | 0.999766 |
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Target: 5'- cGGGUCUGgcgcgggGAcgCggggggcggcggCCGgGUCGGGa -3' miRNA: 3'- -CCCAGAUaua----UUuaGa-----------GGCgCAGCCC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 145513 | 0.68 | 0.999766 |
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Target: 5'- cGGGgCUGcuccggcUCUgCGCGUCGGGc -3' miRNA: 3'- -CCCaGAUauauuu-AGAgGCGCAGCCC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 219037 | 0.69 | 0.999453 |
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Target: 5'- uGGUCUAcGUc-GUCggCCGCGgcgCGGGg -3' miRNA: 3'- cCCAGAUaUAuuUAGa-GGCGCa--GCCC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 220164 | 0.7 | 0.998785 |
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Target: 5'- gGGcGUCgug------CUCUGCGUCGGGa -3' miRNA: 3'- -CC-CAGauauauuuaGAGGCGCAGCCC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 220015 | 0.71 | 0.996154 |
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Target: 5'- cGGGUCgagaggccgCUCCGUcUCGGGg -3' miRNA: 3'- -CCCAGauauauuuaGAGGCGcAGCCC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 166612 | 0.73 | 0.983318 |
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Target: 5'- gGGGUCccaggg---CUCCGCGUcCGGGa -3' miRNA: 3'- -CCCAGauauauuuaGAGGCGCA-GCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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