Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9363 | 5' | -49.1 | NC_002512.2 | + | 214520 | 0.66 | 0.999851 |
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Target: 5'- -aCCUCGAcAUGuUCUaucGGAUCGGggUCa -3' miRNA: 3'- ggGGGGUU-UAC-AGA---UCUAGCCuuAGg -5' |
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| 9363 | 5' | -49.1 | NC_002512.2 | + | 81895 | 0.66 | 0.999884 |
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Target: 5'- cCCUCCCGGA-GUCaAGAaacgUCGGAuagaCCg -3' miRNA: 3'- -GGGGGGUUUaCAGaUCU----AGCCUua--GG- -5' |
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| 9363 | 5' | -49.1 | NC_002512.2 | + | 98152 | 0.66 | 0.999884 |
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Target: 5'- cCCgCCCCGgcggucgccGcgGUCUGGA-CGGGcgcggcgaccGUCCg -3' miRNA: 3'- -GG-GGGGU---------UuaCAGAUCUaGCCU----------UAGG- -5' |
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| 9363 | 5' | -49.1 | NC_002512.2 | + | 180009 | 0.66 | 0.99991 |
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Target: 5'- aUCCCCCAGcgGgaCUcGAucacgcUCGGGAUCUc -3' miRNA: 3'- -GGGGGGUUuaCa-GAuCU------AGCCUUAGG- -5' |
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| 9363 | 5' | -49.1 | NC_002512.2 | + | 195362 | 0.66 | 0.99991 |
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Target: 5'- -aCCCCGGGUG-CUGG-UCGGcugCCu -3' miRNA: 3'- ggGGGGUUUACaGAUCuAGCCuuaGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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