Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9365 | 3' | -52 | NC_002512.2 | + | 143592 | 0.66 | 0.997496 |
Target: 5'- --gGAggCGCCCUUGCGUG-CGGGcCc -3' miRNA: 3'- ggaCUuaGUGGGAGUGCAUaGCCCuG- -5' |
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9365 | 3' | -52 | NC_002512.2 | + | 218601 | 0.66 | 0.997238 |
Target: 5'- cCCgGggUCucGCCCgcggacucgaaggaCGCGcGUCGGGACc -3' miRNA: 3'- -GGaCuuAG--UGGGa-------------GUGCaUAGCCCUG- -5' |
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9365 | 3' | -52 | NC_002512.2 | + | 78030 | 0.66 | 0.997054 |
Target: 5'- --cGAGUCAUCCggUCGCGUGUCcgacacgcGGGCa -3' miRNA: 3'- ggaCUUAGUGGG--AGUGCAUAGc-------CCUG- -5' |
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9365 | 3' | -52 | NC_002512.2 | + | 92981 | 0.66 | 0.997054 |
Target: 5'- gCUGAcGUCGCCCgacCugGUGgccgucUCGGuGGCg -3' miRNA: 3'- gGACU-UAGUGGGa--GugCAU------AGCC-CUG- -5' |
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9365 | 3' | -52 | NC_002512.2 | + | 199381 | 0.66 | 0.997054 |
Target: 5'- gCUGccUCACCUUCACGaacCGGuGGCg -3' miRNA: 3'- gGACuuAGUGGGAGUGCauaGCC-CUG- -5' |
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9365 | 3' | -52 | NC_002512.2 | + | 227442 | 0.66 | 0.996548 |
Target: 5'- gCCgcGGUCACCUUCGCGcUGcCGGGGg -3' miRNA: 3'- -GGacUUAGUGGGAGUGC-AUaGCCCUg -5' |
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9365 | 3' | -52 | NC_002512.2 | + | 155663 | 0.66 | 0.995973 |
Target: 5'- gCCgu--UCGCCC-CGCG--UCGGGACc -3' miRNA: 3'- -GGacuuAGUGGGaGUGCauAGCCCUG- -5' |
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9365 | 3' | -52 | NC_002512.2 | + | 183956 | 0.66 | 0.997496 |
Target: 5'- cCCggGAcgCGCgCUCggGCGUcgucgccuGUCGGGGCg -3' miRNA: 3'- -GGa-CUuaGUGgGAG--UGCA--------UAGCCCUG- -5' |
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9365 | 3' | -52 | NC_002512.2 | + | 214872 | 0.67 | 0.992839 |
Target: 5'- aCCUGGccuacGUCugCCUCAgccCGgacaagcugCGGGACu -3' miRNA: 3'- -GGACU-----UAGugGGAGU---GCaua------GCCCUG- -5' |
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9365 | 3' | -52 | NC_002512.2 | + | 75513 | 0.67 | 0.992839 |
Target: 5'- --cGGAUUACCCUCgACGUugaCGGGcACc -3' miRNA: 3'- ggaCUUAGUGGGAG-UGCAua-GCCC-UG- -5' |
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9365 | 3' | -52 | NC_002512.2 | + | 207970 | 0.67 | 0.993762 |
Target: 5'- ----cGUCGCCCUC-CGUAgCGGcGACg -3' miRNA: 3'- ggacuUAGUGGGAGuGCAUaGCC-CUG- -5' |
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9365 | 3' | -52 | NC_002512.2 | + | 141777 | 0.67 | 0.994587 |
Target: 5'- cCCUGAccgugAUCAgCCC-CGCGgUGUCGGcGAUc -3' miRNA: 3'- -GGACU-----UAGU-GGGaGUGC-AUAGCC-CUG- -5' |
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9365 | 3' | -52 | NC_002512.2 | + | 190869 | 0.67 | 0.994587 |
Target: 5'- aCCUGcggggggCuuuCCC-CGCGUcgCGGGGCg -3' miRNA: 3'- -GGACuua----Gu--GGGaGUGCAuaGCCCUG- -5' |
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9365 | 3' | -52 | NC_002512.2 | + | 225374 | 0.68 | 0.98927 |
Target: 5'- uCCUccucCACCCUCGCGUccAUCcucuucgGGGACg -3' miRNA: 3'- -GGAcuuaGUGGGAGUGCA--UAG-------CCCUG- -5' |
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9365 | 3' | -52 | NC_002512.2 | + | 164048 | 0.68 | 0.98801 |
Target: 5'- uCCUGGAgcugUACUCUCGCGgcgagAUCcGGGCc -3' miRNA: 3'- -GGACUUa---GUGGGAGUGCa----UAGcCCUG- -5' |
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9365 | 3' | -52 | NC_002512.2 | + | 225635 | 0.68 | 0.982968 |
Target: 5'- aCCUGuAUCcCCCgaacCACccGUCGGGGCu -3' miRNA: 3'- -GGACuUAGuGGGa---GUGcaUAGCCCUG- -5' |
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9365 | 3' | -52 | NC_002512.2 | + | 130862 | 0.69 | 0.97881 |
Target: 5'- --cGAggCGCCCUCGCcgacgcgcccUAUCGGGGCc -3' miRNA: 3'- ggaCUuaGUGGGAGUGc---------AUAGCCCUG- -5' |
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9365 | 3' | -52 | NC_002512.2 | + | 194305 | 0.7 | 0.9652 |
Target: 5'- aCCcGAcagGUCACCUUCcuCGUGgccCGGGACg -3' miRNA: 3'- -GGaCU---UAGUGGGAGu-GCAUa--GCCCUG- -5' |
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9365 | 3' | -52 | NC_002512.2 | + | 211885 | 0.72 | 0.921862 |
Target: 5'- cCCUGGagcggGUCACCUUCcggggcugccGCGUcgucgagcGUCGGGACc -3' miRNA: 3'- -GGACU-----UAGUGGGAG----------UGCA--------UAGCCCUG- -5' |
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9365 | 3' | -52 | NC_002512.2 | + | 203847 | 0.73 | 0.870607 |
Target: 5'- uCUUcAAUCACCCUCAUGUgaucaaugaauGUCGcGGACg -3' miRNA: 3'- -GGAcUUAGUGGGAGUGCA-----------UAGC-CCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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