Results 1 - 20 of 60 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9374 | 3' | -53.9 | NC_002512.2 | + | 138653 | 0.66 | 0.989793 |
Target: 5'- aCGCGGcgucccugACgAGCACGGUgGAcUCGCUg -3' miRNA: 3'- gGUGCC--------UG-UCGUGUCAgCUaAGCGGu -5' |
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9374 | 3' | -53.9 | NC_002512.2 | + | 182211 | 0.66 | 0.989793 |
Target: 5'- cCCGggUGGaACAGCACGuUCGGcggCGCCAc -3' miRNA: 3'- -GGU--GCC-UGUCGUGUcAGCUaa-GCGGU- -5' |
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9374 | 3' | -53.9 | NC_002512.2 | + | 168974 | 0.66 | 0.989404 |
Target: 5'- uUCGCGGuCGGUguccgcucgaccgaGCGGUCGcg-CGCCGg -3' miRNA: 3'- -GGUGCCuGUCG--------------UGUCAGCuaaGCGGU- -5' |
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9374 | 3' | -53.9 | NC_002512.2 | + | 219487 | 0.66 | 0.988453 |
Target: 5'- cCCugGGGCGGguCAGaCGAggaugcUgGCCGg -3' miRNA: 3'- -GGugCCUGUCguGUCaGCUa-----AgCGGU- -5' |
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9374 | 3' | -53.9 | NC_002512.2 | + | 159392 | 0.66 | 0.988453 |
Target: 5'- cCCGCGGcguCGGgGCGGaaGGUccggUCGCCAa -3' miRNA: 3'- -GGUGCCu--GUCgUGUCagCUA----AGCGGU- -5' |
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9374 | 3' | -53.9 | NC_002512.2 | + | 112951 | 0.66 | 0.988453 |
Target: 5'- cCCGCGGACcgGGUcgaAGUCGAa--GCCGg -3' miRNA: 3'- -GGUGCCUG--UCGug-UCAGCUaagCGGU- -5' |
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9374 | 3' | -53.9 | NC_002512.2 | + | 92544 | 0.66 | 0.988453 |
Target: 5'- gCCGCGGcggccGCGGCgACGacGUCGGaggCGCCGa -3' miRNA: 3'- -GGUGCC-----UGUCG-UGU--CAGCUaa-GCGGU- -5' |
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9374 | 3' | -53.9 | NC_002512.2 | + | 185850 | 0.66 | 0.988453 |
Target: 5'- uUCugGGAgCGGUAC-GUCGAggagggCGCCu -3' miRNA: 3'- -GGugCCU-GUCGUGuCAGCUaa----GCGGu -5' |
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9374 | 3' | -53.9 | NC_002512.2 | + | 152351 | 0.66 | 0.986981 |
Target: 5'- aCCGCGGACcuccgAGCACGGagacCGGccgUgGCCGg -3' miRNA: 3'- -GGUGCCUG-----UCGUGUCa---GCUa--AgCGGU- -5' |
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9374 | 3' | -53.9 | NC_002512.2 | + | 139570 | 0.66 | 0.986981 |
Target: 5'- aCCugGGACAGCGCuaaccUCcGUUCGgCu -3' miRNA: 3'- -GGugCCUGUCGUGuc---AGcUAAGCgGu -5' |
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9374 | 3' | -53.9 | NC_002512.2 | + | 149257 | 0.66 | 0.986981 |
Target: 5'- gCCGCGGACGGCcggACGGguacgaCGAggagCGCgAg -3' miRNA: 3'- -GGUGCCUGUCG---UGUCa-----GCUaa--GCGgU- -5' |
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9374 | 3' | -53.9 | NC_002512.2 | + | 169344 | 0.66 | 0.986981 |
Target: 5'- gCCgACGGAUAcGCGuuuGUCGAgcagaUCGCCAg -3' miRNA: 3'- -GG-UGCCUGU-CGUgu-CAGCUa----AGCGGU- -5' |
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9374 | 3' | -53.9 | NC_002512.2 | + | 195057 | 0.66 | 0.986981 |
Target: 5'- cCCGCGGGCGGgAgGGUCGuccgGUCGg -3' miRNA: 3'- -GGUGCCUGUCgUgUCAGCuaagCGGU- -5' |
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9374 | 3' | -53.9 | NC_002512.2 | + | 214954 | 0.66 | 0.985537 |
Target: 5'- gCCGCGGAC-GCACGGgguccggauccagacCGA--CGCCGa -3' miRNA: 3'- -GGUGCCUGuCGUGUCa--------------GCUaaGCGGU- -5' |
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9374 | 3' | -53.9 | NC_002512.2 | + | 189119 | 0.66 | 0.985369 |
Target: 5'- gCC-CGGACGcGCgAUAGg-GGUUCGCCGg -3' miRNA: 3'- -GGuGCCUGU-CG-UGUCagCUAAGCGGU- -5' |
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9374 | 3' | -53.9 | NC_002512.2 | + | 161518 | 0.66 | 0.985369 |
Target: 5'- gCGCGGACGGCACcGcCGA---GCCc -3' miRNA: 3'- gGUGCCUGUCGUGuCaGCUaagCGGu -5' |
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9374 | 3' | -53.9 | NC_002512.2 | + | 101432 | 0.66 | 0.985369 |
Target: 5'- gCgGCGGccgGCGGCGCGG-CGA--CGCCGa -3' miRNA: 3'- -GgUGCC---UGUCGUGUCaGCUaaGCGGU- -5' |
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9374 | 3' | -53.9 | NC_002512.2 | + | 214752 | 0.66 | 0.983608 |
Target: 5'- gCGCGGaACAGCgugccgucGCAGUCcgg-CGCCGg -3' miRNA: 3'- gGUGCC-UGUCG--------UGUCAGcuaaGCGGU- -5' |
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9374 | 3' | -53.9 | NC_002512.2 | + | 184889 | 0.66 | 0.983608 |
Target: 5'- gCCACGGGguCAGCggGCGG-CGGcgcuacugccccUUCGCCGa -3' miRNA: 3'- -GGUGCCU--GUCG--UGUCaGCU------------AAGCGGU- -5' |
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9374 | 3' | -53.9 | NC_002512.2 | + | 123169 | 0.66 | 0.98305 |
Target: 5'- -gGCGGACaggAGCGCGGUC-AUcccggagagccccaUCGCCGa -3' miRNA: 3'- ggUGCCUG---UCGUGUCAGcUA--------------AGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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