Results 41 - 48 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9381 | 3' | -55.1 | NC_002512.2 | + | 165470 | 0.66 | 0.973059 |
Target: 5'- -cGAUcgGaagaUCCCGAguuucgggUGGCUGCGCGUg -3' miRNA: 3'- cuCUAuaCc---AGGGCU--------ACUGGCGCGCG- -5' |
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9381 | 3' | -55.1 | NC_002512.2 | + | 207080 | 0.66 | 0.973059 |
Target: 5'- cGAGAaGUGGaUCCCGcccuuGUGGuaGCGgGCg -3' miRNA: 3'- -CUCUaUACC-AGGGC-----UACUggCGCgCG- -5' |
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9381 | 3' | -55.1 | NC_002512.2 | + | 110678 | 0.66 | 0.975606 |
Target: 5'- ------cGGUCCUGGUGcUCGCGCaGCc -3' miRNA: 3'- cucuauaCCAGGGCUACuGGCGCG-CG- -5' |
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9381 | 3' | -55.1 | NC_002512.2 | + | 150731 | 0.66 | 0.975606 |
Target: 5'- -----uUGGacuUCCCGucgGcCCGCGCGCg -3' miRNA: 3'- cucuauACC---AGGGCua-CuGGCGCGCG- -5' |
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9381 | 3' | -55.1 | NC_002512.2 | + | 105054 | 0.66 | 0.975606 |
Target: 5'- ------aGGUCCCGGUGuCCgGCaCGCa -3' miRNA: 3'- cucuauaCCAGGGCUACuGG-CGcGCG- -5' |
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9381 | 3' | -55.1 | NC_002512.2 | + | 169973 | 0.66 | 0.977744 |
Target: 5'- aGGGUcgGGgcgaacgUCCCGcucGCCGUGCGCc -3' miRNA: 3'- cUCUAuaCC-------AGGGCuacUGGCGCGCG- -5' |
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9381 | 3' | -55.1 | NC_002512.2 | + | 182679 | 0.66 | 0.977972 |
Target: 5'- -uGAUGcGGUUCUGG-GGCCGgGCGUc -3' miRNA: 3'- cuCUAUaCCAGGGCUaCUGGCgCGCG- -5' |
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9381 | 3' | -55.1 | NC_002512.2 | + | 226799 | 0.66 | 0.977972 |
Target: 5'- ----cGUGGUCUCGGccgccgccgUGGCCGC-CGCc -3' miRNA: 3'- cucuaUACCAGGGCU---------ACUGGCGcGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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