Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9591 | 5' | -53.8 | NC_002577.1 | + | 102399 | 0.66 | 0.971149 |
Target: 5'- --gUUGGGAgAGauUCGGGACgGGACAGa -3' miRNA: 3'- caaGAUCCUgUU--GGCCCUG-CCUGUCc -5' |
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9591 | 5' | -53.8 | NC_002577.1 | + | 64443 | 0.66 | 0.973893 |
Target: 5'- --aCUGGGGCAACauaauGGGugGGAgAa- -3' miRNA: 3'- caaGAUCCUGUUGg----CCCugCCUgUcc -5' |
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9591 | 5' | -53.8 | NC_002577.1 | + | 75946 | 0.66 | 0.973893 |
Target: 5'- --aCUAGGGCAugCGaaaaGAUGGcCAGGc -3' miRNA: 3'- caaGAUCCUGUugGCc---CUGCCuGUCC- -5' |
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9591 | 5' | -53.8 | NC_002577.1 | + | 76896 | 0.66 | 0.976444 |
Target: 5'- --gCUGGaGAUAACaGGGACGGGCu-- -3' miRNA: 3'- caaGAUC-CUGUUGgCCCUGCCUGucc -5' |
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9591 | 5' | -53.8 | NC_002577.1 | + | 122997 | 0.65 | 0.978578 |
Target: 5'- cGUUCUcgugcgagggaucGGGACGAuCCGcGGACGagaacGAUGGGg -3' miRNA: 3'- -CAAGA-------------UCCUGUU-GGC-CCUGC-----CUGUCC- -5' |
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9591 | 5' | -53.8 | NC_002577.1 | + | 10617 | 0.65 | 0.978578 |
Target: 5'- cGUUCUcgugcgagggaucGGGACGAuCCGcGGACGagaacGAUGGGg -3' miRNA: 3'- -CAAGA-------------UCCUGUU-GGC-CCUGC-----CUGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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