Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9591 | 5' | -53.8 | NC_002577.1 | + | 128302 | 1.12 | 0.003787 |
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Target: 5'- cGUUCUAGGACAACCGGGACGGACAGGg -3' miRNA: 3'- -CAAGAUCCUGUUGGCCCUGCCUGUCC- -5' |
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| 9591 | 5' | -53.8 | NC_002577.1 | + | 128458 | 0.72 | 0.75742 |
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Target: 5'- -cUCUAGGGgAcCCGGGAaGGACuGGa -3' miRNA: 3'- caAGAUCCUgUuGGCCCUgCCUGuCC- -5' |
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| 9591 | 5' | -53.8 | NC_002577.1 | + | 130206 | 0.68 | 0.936748 |
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Target: 5'- cUUCUcGGGCAccgcACCGaaGGAUGGAguGGg -3' miRNA: 3'- cAAGAuCCUGU----UGGC--CCUGCCUguCC- -5' |
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| 9591 | 5' | -53.8 | NC_002577.1 | + | 130458 | 0.67 | 0.958097 |
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Target: 5'- -gUCU-GGACuuuACCGGGGCGGcuucgucccGCGGc -3' miRNA: 3'- caAGAuCCUGu--UGGCCCUGCC---------UGUCc -5' |
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| 9591 | 5' | -53.8 | NC_002577.1 | + | 131707 | 0.67 | 0.953895 |
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Target: 5'- aGggUUGGGGCccguaaggguaggGGCUGGGucuguuACGGGCAGGg -3' miRNA: 3'- -CaaGAUCCUG-------------UUGGCCC------UGCCUGUCC- -5' |
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| 9591 | 5' | -53.8 | NC_002577.1 | + | 146241 | 0.66 | 0.965049 |
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Target: 5'- ---aUGGGAUuACCGGaaGACGaGAUAGGg -3' miRNA: 3'- caagAUCCUGuUGGCC--CUGC-CUGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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