Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9595 | 3' | -48.5 | NC_002577.1 | + | 123068 | 0.66 | 0.999824 |
Target: 5'- -aGUCGGGgggcgacccgcgACCGuGUUCCGCGagCGGc -3' miRNA: 3'- ggCAGCCU------------UGGCuUAAGGUGUaaGCU- -5' |
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9595 | 3' | -48.5 | NC_002577.1 | + | 10546 | 0.66 | 0.999824 |
Target: 5'- -aGUCGGGgggcgacccgcgACCGuGUUCCGCGagCGGc -3' miRNA: 3'- ggCAGCCU------------UGGCuUAAGGUGUaaGCU- -5' |
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9595 | 3' | -48.5 | NC_002577.1 | + | 118481 | 0.66 | 0.999716 |
Target: 5'- cCUGUaUGGAACCG--UUCCGaGUUCGGg -3' miRNA: 3'- -GGCA-GCCUUGGCuuAAGGUgUAAGCU- -5' |
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9595 | 3' | -48.5 | NC_002577.1 | + | 134619 | 0.66 | 0.999709 |
Target: 5'- gCCGUCGGGAUccugcaaCGAGcUCCGCuccgUGAa -3' miRNA: 3'- -GGCAGCCUUG-------GCUUaAGGUGuaa-GCU- -5' |
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9595 | 3' | -48.5 | NC_002577.1 | + | 162708 | 0.66 | 0.999709 |
Target: 5'- gCCGUCGGGAUccugcaaCGAGcUCCGCuccgUGAa -3' miRNA: 3'- -GGCAGCCUUG-------GCUUaAGGUGuaa-GCU- -5' |
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9595 | 3' | -48.5 | NC_002577.1 | + | 27815 | 0.66 | 0.999642 |
Target: 5'- gCGUCGGuuACUGAAU-CCGgAUUCa- -3' miRNA: 3'- gGCAGCCu-UGGCUUAaGGUgUAAGcu -5' |
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9595 | 3' | -48.5 | NC_002577.1 | + | 132846 | 0.66 | 0.999617 |
Target: 5'- aCGcUCGGGGCCGggUacaaguugcccgggUCCGCGggUCuGAa -3' miRNA: 3'- gGC-AGCCUUGGCuuA--------------AGGUGUa-AG-CU- -5' |
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9595 | 3' | -48.5 | NC_002577.1 | + | 767 | 0.66 | 0.999617 |
Target: 5'- aCGcUCGGGGCCGggUacaaguugcccgggUCCGCGggUCuGAa -3' miRNA: 3'- gGC-AGCCUUGGCuuA--------------AGGUGUa-AG-CU- -5' |
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9595 | 3' | -48.5 | NC_002577.1 | + | 78429 | 0.67 | 0.999314 |
Target: 5'- cCCGUCGGAcUCGAAUUgCCGac--CGAu -3' miRNA: 3'- -GGCAGCCUuGGCUUAA-GGUguaaGCU- -5' |
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9595 | 3' | -48.5 | NC_002577.1 | + | 122846 | 0.67 | 0.999314 |
Target: 5'- gCGUCGGcgacgcGGCCGAAcgCCGCucgCGGa -3' miRNA: 3'- gGCAGCC------UUGGCUUaaGGUGuaaGCU- -5' |
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9595 | 3' | -48.5 | NC_002577.1 | + | 10767 | 0.67 | 0.999314 |
Target: 5'- gCGUCGGcgacgcGGCCGAAcgCCGCucgCGGa -3' miRNA: 3'- gGCAGCC------UUGGCUUaaGGUGuaaGCU- -5' |
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9595 | 3' | -48.5 | NC_002577.1 | + | 122237 | 0.67 | 0.999158 |
Target: 5'- cCCGUCGGGAgcgaucCCGGAUcggacgCCGCgg-CGAa -3' miRNA: 3'- -GGCAGCCUU------GGCUUAa-----GGUGuaaGCU- -5' |
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9595 | 3' | -48.5 | NC_002577.1 | + | 11377 | 0.67 | 0.999158 |
Target: 5'- cCCGUCGGGAgcgaucCCGGAUcggacgCCGCgg-CGAa -3' miRNA: 3'- -GGCAGCCUU------GGCUUAa-----GGUGuaaGCU- -5' |
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9595 | 3' | -48.5 | NC_002577.1 | + | 42871 | 0.68 | 0.998752 |
Target: 5'- gCCGUCGGuACCGAAaauaaaCCGCcagCGGu -3' miRNA: 3'- -GGCAGCCuUGGCUUaa----GGUGuaaGCU- -5' |
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9595 | 3' | -48.5 | NC_002577.1 | + | 5587 | 0.68 | 0.998493 |
Target: 5'- gCCGcUCGGAccGCUGcGAUUCCAUccUUCGAc -3' miRNA: 3'- -GGC-AGCCU--UGGC-UUAAGGUGu-AAGCU- -5' |
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9595 | 3' | -48.5 | NC_002577.1 | + | 128027 | 0.68 | 0.998493 |
Target: 5'- gCCGcUCGGAccGCUGcGAUUCCAUccUUCGAc -3' miRNA: 3'- -GGC-AGCCU--UGGC-UUAAGGUGu-AAGCU- -5' |
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9595 | 3' | -48.5 | NC_002577.1 | + | 158395 | 0.68 | 0.997839 |
Target: 5'- gCCGUgGGGcgucccGCCGggUUCCGgAUUUc- -3' miRNA: 3'- -GGCAgCCU------UGGCuuAAGGUgUAAGcu -5' |
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9595 | 3' | -48.5 | NC_002577.1 | + | 138942 | 0.68 | 0.997839 |
Target: 5'- gCCGUgGGGcgucccGCCGggUUCCGgAUUUc- -3' miRNA: 3'- -GGCAgCCU------UGGCuuAAGGUgUAAGcu -5' |
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9595 | 3' | -48.5 | NC_002577.1 | + | 22087 | 0.69 | 0.996962 |
Target: 5'- gCGUCGGGucGCCcacgcaaacGAAUucuuuggagUCCACAUUUGAg -3' miRNA: 3'- gGCAGCCU--UGG---------CUUA---------AGGUGUAAGCU- -5' |
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9595 | 3' | -48.5 | NC_002577.1 | + | 125017 | 0.69 | 0.996423 |
Target: 5'- -gGUCGGAACucgCGAcUUCCACggUCGc -3' miRNA: 3'- ggCAGCCUUG---GCUuAAGGUGuaAGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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