Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9595 | 3' | -48.5 | NC_002577.1 | + | 8597 | 0.69 | 0.996423 |
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Target: 5'- -gGUCGGAACucgCGAcUUCCACggUCGc -3' miRNA: 3'- ggCAGCCUUG---GCUuAAGGUGuaAGCu -5' |
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| 9595 | 3' | -48.5 | NC_002577.1 | + | 21337 | 0.69 | 0.996423 |
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Target: 5'- cCUGUUGGcugCGAAUUUCACAUUUGGc -3' miRNA: 3'- -GGCAGCCuugGCUUAAGGUGUAAGCU- -5' |
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| 9595 | 3' | -48.5 | NC_002577.1 | + | 8505 | 0.69 | 0.99511 |
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Target: 5'- gCgGcCGGGACgGGAUUCCugAUUCc- -3' miRNA: 3'- -GgCaGCCUUGgCUUAAGGugUAAGcu -5' |
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| 9595 | 3' | -48.5 | NC_002577.1 | + | 125109 | 0.69 | 0.99511 |
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Target: 5'- gCgGcCGGGACgGGAUUCCugAUUCc- -3' miRNA: 3'- -GgCaGCCUUGgCUUAAGGugUAAGcu -5' |
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| 9595 | 3' | -48.5 | NC_002577.1 | + | 56479 | 0.69 | 0.995035 |
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Target: 5'- aCCGUCGGAGCCG--UUCUggcacuaACGgaCGAc -3' miRNA: 3'- -GGCAGCCUUGGCuuAAGG-------UGUaaGCU- -5' |
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| 9595 | 3' | -48.5 | NC_002577.1 | + | 27267 | 0.73 | 0.954612 |
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Target: 5'- aCGUCGGAACCGGAg-CCGCGg---- -3' miRNA: 3'- gGCAGCCUUGGCUUaaGGUGUaagcu -5' |
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| 9595 | 3' | -48.5 | NC_002577.1 | + | 136992 | 0.79 | 0.730965 |
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Target: 5'- gCCGUCGaGAACCcGcgUCCGCGUUUGGg -3' miRNA: 3'- -GGCAGC-CUUGGcUuaAGGUGUAAGCU- -5' |
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| 9595 | 3' | -48.5 | NC_002577.1 | + | 160346 | 0.79 | 0.730965 |
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Target: 5'- gCCGUCGaGAACCcGcgUCCGCGUUUGGg -3' miRNA: 3'- -GGCAGC-CUUGGcUuaAGGUGUAAGCU- -5' |
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| 9595 | 3' | -48.5 | NC_002577.1 | + | 124880 | 1.13 | 0.012772 |
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Target: 5'- cCCGUCGGAACCGAAUUCCACAUUCGAg -3' miRNA: 3'- -GGCAGCCUUGGCUUAAGGUGUAAGCU- -5' |
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| 9595 | 3' | -48.5 | NC_002577.1 | + | 8733 | 1.13 | 0.012772 |
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Target: 5'- cCCGUCGGAACCGAAUUCCACAUUCGAg -3' miRNA: 3'- -GGCAGCCUUGGCUUAAGGUGUAAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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