Results 1 - 20 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9596 | 5' | -53.4 | NC_002577.1 | + | 28379 | 0.66 | 0.976789 |
Target: 5'- uGGUACGCGCua-GUGAaaucUCGCUuggaCGCGg -3' miRNA: 3'- -CCGUGCGCGuagUAUUc---AGCGA----GCGC- -5' |
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9596 | 5' | -53.4 | NC_002577.1 | + | 124446 | 0.66 | 0.976789 |
Target: 5'- uGGCGCGgGCGggg-GAGUCcgUCGCGu -3' miRNA: 3'- -CCGUGCgCGUaguaUUCAGcgAGCGC- -5' |
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9596 | 5' | -53.4 | NC_002577.1 | + | 9168 | 0.66 | 0.976789 |
Target: 5'- uGGCGCGgGCGggg-GAGUCcgUCGCGu -3' miRNA: 3'- -CCGUGCgCGUaguaUUCAGcgAGCGC- -5' |
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9596 | 5' | -53.4 | NC_002577.1 | + | 1504 | 0.66 | 0.974243 |
Target: 5'- uGUGCGCGCAUg------CGCUCGCGc -3' miRNA: 3'- cCGUGCGCGUAguauucaGCGAGCGC- -5' |
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9596 | 5' | -53.4 | NC_002577.1 | + | 132110 | 0.66 | 0.974243 |
Target: 5'- uGUGCGCGCAUg------CGCUCGCGc -3' miRNA: 3'- cCGUGCGCGUAguauucaGCGAGCGC- -5' |
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9596 | 5' | -53.4 | NC_002577.1 | + | 36483 | 0.66 | 0.9715 |
Target: 5'- uGCGcCGCGCAUUGUGcaucGUCGUacUCGUGa -3' miRNA: 3'- cCGU-GCGCGUAGUAUu---CAGCG--AGCGC- -5' |
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9596 | 5' | -53.4 | NC_002577.1 | + | 48484 | 0.66 | 0.968553 |
Target: 5'- --gACG-GCG-CAUGAGUCGC-CGCGg -3' miRNA: 3'- ccgUGCgCGUaGUAUUCAGCGaGCGC- -5' |
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9596 | 5' | -53.4 | NC_002577.1 | + | 21186 | 0.67 | 0.964073 |
Target: 5'- cGGCAaccuccggauagaGCGgAgCAgaggGAGUCGUUCGCGa -3' miRNA: 3'- -CCGUg------------CGCgUaGUa---UUCAGCGAGCGC- -5' |
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9596 | 5' | -53.4 | NC_002577.1 | + | 33521 | 0.67 | 0.958427 |
Target: 5'- cGGaACGCGUAaCG--AGcCGCUCGCGa -3' miRNA: 3'- -CCgUGCGCGUaGUauUCaGCGAGCGC- -5' |
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9596 | 5' | -53.4 | NC_002577.1 | + | 30524 | 0.67 | 0.958427 |
Target: 5'- aGGCGCGCGCAcUAcAAGUacauugcgCGCUCuCGa -3' miRNA: 3'- -CCGUGCGCGUaGUaUUCA--------GCGAGcGC- -5' |
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9596 | 5' | -53.4 | NC_002577.1 | + | 53875 | 0.67 | 0.958427 |
Target: 5'- uGGCGCuuGCGuCAUCAUGAGUUGaagaCUUGUa -3' miRNA: 3'- -CCGUG--CGC-GUAGUAUUCAGC----GAGCGc -5' |
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9596 | 5' | -53.4 | NC_002577.1 | + | 155312 | 0.67 | 0.954605 |
Target: 5'- cGCACGaacCGCAc----AGUCGCUCGCa -3' miRNA: 3'- cCGUGC---GCGUaguauUCAGCGAGCGc -5' |
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9596 | 5' | -53.4 | NC_002577.1 | + | 102000 | 0.67 | 0.95421 |
Target: 5'- gGGCcaaaaauACG-GUAUCA-AAGUCGCUCGgGg -3' miRNA: 3'- -CCG-------UGCgCGUAGUaUUCAGCGAGCgC- -5' |
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9596 | 5' | -53.4 | NC_002577.1 | + | 156153 | 0.67 | 0.950552 |
Target: 5'- --gGCGCGCAUCAgGGGUCucCUgGCGa -3' miRNA: 3'- ccgUGCGCGUAGUaUUCAGc-GAgCGC- -5' |
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9596 | 5' | -53.4 | NC_002577.1 | + | 141185 | 0.67 | 0.950552 |
Target: 5'- --gGCGCGCAUCAgGGGUCucCUgGCGa -3' miRNA: 3'- ccgUGCGCGUAGUaUUCAGc-GAgCGC- -5' |
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9596 | 5' | -53.4 | NC_002577.1 | + | 20829 | 0.67 | 0.950552 |
Target: 5'- aGCAUGCGCGgaggaAUGcuaUCGCUCGCu -3' miRNA: 3'- cCGUGCGCGUag---UAUuc-AGCGAGCGc -5' |
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9596 | 5' | -53.4 | NC_002577.1 | + | 31950 | 0.67 | 0.948008 |
Target: 5'- aGCACGCugugacagGCAgugCGUAAGUUGCcggcuccuacgcccaUCGCGa -3' miRNA: 3'- cCGUGCG--------CGUa--GUAUUCAGCG---------------AGCGC- -5' |
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9596 | 5' | -53.4 | NC_002577.1 | + | 154706 | 0.67 | 0.944932 |
Target: 5'- cGGCAcCGCGCuccugguaggugcuGUCG--GGUCGCgcgaUCGCGu -3' miRNA: 3'- -CCGU-GCGCG--------------UAGUauUCAGCG----AGCGC- -5' |
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9596 | 5' | -53.4 | NC_002577.1 | + | 142630 | 0.67 | 0.944932 |
Target: 5'- cGGCAcCGCGCuccugguaggugcuGUCG--GGUCGCgcgaUCGCGu -3' miRNA: 3'- -CCGU-GCGCG--------------UAGUauUCAGCG----AGCGC- -5' |
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9596 | 5' | -53.4 | NC_002577.1 | + | 7964 | 0.68 | 0.941739 |
Target: 5'- gGGCGCGgGCAgg--GAGUUaaCUCGCGa -3' miRNA: 3'- -CCGUGCgCGUaguaUUCAGc-GAGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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