Results 21 - 28 of 28 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | + | 119184 | 1.01 | 0.036813 |
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Target: 5'- uUUACGCAGCUGCGUAUACCCAGc -3' miRNA: 3'- -AAUGCGUCGACGCAUAUGGGUC- -5' |
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| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | + | 119226 | 0.84 | 0.355563 |
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Target: 5'- uUUACGCAGCUGgGUAgACCCAGc -3' miRNA: 3'- -AAUGCGUCGACgCAUaUGGGUC- -5' |
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| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | - | 119337 | 0.9 | 0.15678 |
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Target: 5'- aUACGCAGCUGCGUAaACCCGGc -3' miRNA: 3'- aAUGCGUCGACGCAUaUGGGUC- -5' |
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| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | - | 119393 | 0.71 | 0.955263 |
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Target: 5'- aUACcCAGCUGgGUcUACCCAGc -3' miRNA: 3'- aAUGcGUCGACgCAuAUGGGUC- -5' |
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| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | + | 120311 | 0.75 | 0.798462 |
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Target: 5'- aUGCGCGGCUGCGUGguauCUCAu -3' miRNA: 3'- aAUGCGUCGACGCAUau--GGGUc -5' |
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| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | - | 121970 | 0.7 | 0.963459 |
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Target: 5'- uUUACGCAgGgUGCGgugcUGCCCAGg -3' miRNA: 3'- -AAUGCGU-CgACGCau--AUGGGUC- -5' |
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| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | + | 128044 | 0.69 | 0.979241 |
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Target: 5'- -gGCGCGGUUGgGgGUGCCCGc -3' miRNA: 3'- aaUGCGUCGACgCaUAUGGGUc -5' |
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| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | - | 130917 | 0.68 | 0.992032 |
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Target: 5'- -gACGguGCUGCccagcuggGCCCAGg -3' miRNA: 3'- aaUGCguCGACGcaua----UGGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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