Results 21 - 28 of 28 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | + | 86131 | 0.68 | 0.990742 |
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Target: 5'- --uUGCGGCUGUG-GUugCCAGg -3' miRNA: 3'- aauGCGUCGACGCaUAugGGUC- -5' |
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| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | + | 55152 | 0.66 | 0.998673 |
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Target: 5'- ---aGCAGCacUGCGguUACCCGGg -3' miRNA: 3'- aaugCGUCG--ACGCauAUGGGUC- -5' |
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| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | + | 47330 | 0.67 | 0.995068 |
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Target: 5'- cUGCaGCAGCUGUugGUGUACCaCAu -3' miRNA: 3'- aAUG-CGUCGACG--CAUAUGG-GUc -5' |
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| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | + | 26365 | 0.73 | 0.896306 |
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Target: 5'- -gGCGCAGggGUGUAUGCCCu- -3' miRNA: 3'- aaUGCGUCgaCGCAUAUGGGuc -5' |
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| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | + | 23658 | 0.76 | 0.734341 |
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Target: 5'- -aACcCAGCUGgGUAUACCCAGc -3' miRNA: 3'- aaUGcGUCGACgCAUAUGGGUC- -5' |
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| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | + | 23617 | 0.68 | 0.989293 |
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Target: 5'- aUACcCAGCUGgGUAauccaguagguaUACCCAGg -3' miRNA: 3'- aAUGcGUCGACgCAU------------AUGGGUC- -5' |
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| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | + | 19118 | 0.67 | 0.995068 |
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Target: 5'- -cGCGUguuuacAGCaGCGUAUGCCCGc -3' miRNA: 3'- aaUGCG------UCGaCGCAUAUGGGUc -5' |
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| MIMAT0002185 | kshv-miR-K12-9 | -46.8 | NC_003409.1 | + | 10062 | 0.72 | 0.935339 |
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Target: 5'- -cACGCAGCUGCaGUuUGCCUAc -3' miRNA: 3'- aaUGCGUCGACG-CAuAUGGGUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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