Results 1 - 20 of 50 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10296 | 5' | -52 | NC_002687.1 | + | 72724 | 0.66 | 0.999546 |
Target: 5'- gCAGcauCGUCCGUcauaaGCGcCCACgcugaCCCCa -3' miRNA: 3'- gGUUu--GCAGGUAa----CGCaGGUGa----GGGG- -5' |
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10296 | 5' | -52 | NC_002687.1 | + | 38462 | 0.66 | 0.999546 |
Target: 5'- --cGGCaUuuGUUGCGcCCGCUCCCa -3' miRNA: 3'- gguUUGcAggUAACGCaGGUGAGGGg -5' |
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10296 | 5' | -52 | NC_002687.1 | + | 203024 | 0.66 | 0.999546 |
Target: 5'- gUCAAGCGUCCAcgugacuccgUUGgGgucgCCACUCaaCa -3' miRNA: 3'- -GGUUUGCAGGU----------AACgCa---GGUGAGggG- -5' |
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10296 | 5' | -52 | NC_002687.1 | + | 64044 | 0.66 | 0.999536 |
Target: 5'- gCCGucuAUGUCCuuacgAUUGaCGUCCGCgccuuguUCCCUg -3' miRNA: 3'- -GGUu--UGCAGG-----UAAC-GCAGGUG-------AGGGG- -5' |
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10296 | 5' | -52 | NC_002687.1 | + | 311349 | 0.66 | 0.999443 |
Target: 5'- cUCGggUGUacaguguaUGUUGUGUCCACUUCCg -3' miRNA: 3'- -GGUuuGCAg-------GUAACGCAGGUGAGGGg -5' |
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10296 | 5' | -52 | NC_002687.1 | + | 330545 | 0.66 | 0.999443 |
Target: 5'- -uGAACGcCCAguUUGCGUCCGCUg--- -3' miRNA: 3'- ggUUUGCaGGU--AACGCAGGUGAgggg -5' |
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10296 | 5' | -52 | NC_002687.1 | + | 136729 | 0.66 | 0.999409 |
Target: 5'- --cAACaUCCAUUGCGUuggaggcguuuaccCCGCUCUCUc -3' miRNA: 3'- gguUUGcAGGUAACGCA--------------GGUGAGGGG- -5' |
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10296 | 5' | -52 | NC_002687.1 | + | 304873 | 0.66 | 0.999321 |
Target: 5'- gCCAGugGaCCGaacucgGgGUCCACaUUCCCg -3' miRNA: 3'- -GGUUugCaGGUaa----CgCAGGUG-AGGGG- -5' |
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10296 | 5' | -52 | NC_002687.1 | + | 173834 | 0.66 | 0.999321 |
Target: 5'- uCCAcuuGCGccUCCAcUUGCGccUCCAgUCCCa -3' miRNA: 3'- -GGUu--UGC--AGGU-AACGC--AGGUgAGGGg -5' |
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10296 | 5' | -52 | NC_002687.1 | + | 173882 | 0.66 | 0.999321 |
Target: 5'- uCCAcuuGCGccUCCAcUUGCGccUCCAgUCCCa -3' miRNA: 3'- -GGUu--UGC--AGGU-AACGC--AGGUgAGGGg -5' |
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10296 | 5' | -52 | NC_002687.1 | + | 154514 | 0.66 | 0.999007 |
Target: 5'- ---uGCGUCUucUGCGUCCuCUCCg- -3' miRNA: 3'- gguuUGCAGGuaACGCAGGuGAGGgg -5' |
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10296 | 5' | -52 | NC_002687.1 | + | 194128 | 0.66 | 0.998989 |
Target: 5'- gUCAAcgcCGUCCGccUGCGcCCGCUcguuuggCCCCg -3' miRNA: 3'- -GGUUu--GCAGGUa-ACGCaGGUGA-------GGGG- -5' |
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10296 | 5' | -52 | NC_002687.1 | + | 165046 | 0.67 | 0.998808 |
Target: 5'- aCGAACGUCCcc-GCGUCUccguCUaCCUCa -3' miRNA: 3'- gGUUUGCAGGuaaCGCAGGu---GA-GGGG- -5' |
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10296 | 5' | -52 | NC_002687.1 | + | 153893 | 0.67 | 0.998808 |
Target: 5'- gCAAACGUCg---GCGUCCACaCCa- -3' miRNA: 3'- gGUUUGCAGguaaCGCAGGUGaGGgg -5' |
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10296 | 5' | -52 | NC_002687.1 | + | 293750 | 0.67 | 0.998576 |
Target: 5'- -aAGAUGaUCU-UUGCGUCCuGCUCCCg -3' miRNA: 3'- ggUUUGC-AGGuAACGCAGG-UGAGGGg -5' |
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10296 | 5' | -52 | NC_002687.1 | + | 86044 | 0.67 | 0.998576 |
Target: 5'- gCCAGACGggCC--UGUGUCacccuuUUCCCCg -3' miRNA: 3'- -GGUUUGCa-GGuaACGCAGgu----GAGGGG- -5' |
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10296 | 5' | -52 | NC_002687.1 | + | 50705 | 0.67 | 0.998576 |
Target: 5'- gCCAca-GUCCg--GCGcugaaagaUCCACUCCCUc -3' miRNA: 3'- -GGUuugCAGGuaaCGC--------AGGUGAGGGG- -5' |
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10296 | 5' | -52 | NC_002687.1 | + | 170632 | 0.67 | 0.998576 |
Target: 5'- uCCGAcaACGagUCGUUGuUGUCCuACUCCUCa -3' miRNA: 3'- -GGUU--UGCa-GGUAAC-GCAGG-UGAGGGG- -5' |
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10296 | 5' | -52 | NC_002687.1 | + | 87990 | 0.67 | 0.998576 |
Target: 5'- -aAGACGUCgAc-GUGUCCAgcgaacucuucCUCCCCg -3' miRNA: 3'- ggUUUGCAGgUaaCGCAGGU-----------GAGGGG- -5' |
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10296 | 5' | -52 | NC_002687.1 | + | 149104 | 0.67 | 0.998576 |
Target: 5'- aCCGucGCGUCCAaUG-GaaCACUCCCUg -3' miRNA: 3'- -GGUu-UGCAGGUaACgCagGUGAGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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