Results 1 - 20 of 57 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10709 | 3' | -59.3 | NC_002794.1 | + | 188939 | 0.66 | 0.808712 |
Target: 5'- aGGCCcgcuGUCC-GGG-CGuGCGCAGGUUc -3' miRNA: 3'- -UUGGu---UAGGuCCCgGCuCGCGUCCAG- -5' |
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10709 | 3' | -59.3 | NC_002794.1 | + | 192399 | 0.66 | 0.808712 |
Target: 5'- cGCCGcgCCGGGGUaguCGAaacgccGCGaCGGGUCc -3' miRNA: 3'- uUGGUuaGGUCCCG---GCU------CGC-GUCCAG- -5' |
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10709 | 3' | -59.3 | NC_002794.1 | + | 29828 | 0.66 | 0.808712 |
Target: 5'- gAGCaGGUCCGGGGUCGugcagGGgGCAGGa- -3' miRNA: 3'- -UUGgUUAGGUCCCGGC-----UCgCGUCCag -5' |
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10709 | 3' | -59.3 | NC_002794.1 | + | 57398 | 0.66 | 0.808712 |
Target: 5'- cACCGGcUCCGGcgcccgcguGGCCGGcGUGCuGGUCg -3' miRNA: 3'- uUGGUU-AGGUC---------CCGGCU-CGCGuCCAG- -5' |
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10709 | 3' | -59.3 | NC_002794.1 | + | 59123 | 0.66 | 0.808712 |
Target: 5'- cGGCCA--CCGaGGCCGAcuucGCGCAGGg- -3' miRNA: 3'- -UUGGUuaGGUcCCGGCU----CGCGUCCag -5' |
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10709 | 3' | -59.3 | NC_002794.1 | + | 25115 | 0.66 | 0.800013 |
Target: 5'- gGACCGGUCgagaucgagCGGGGCaCGcGCGCAcGGUa -3' miRNA: 3'- -UUGGUUAG---------GUCCCG-GCuCGCGU-CCAg -5' |
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10709 | 3' | -59.3 | NC_002794.1 | + | 110280 | 0.66 | 0.791169 |
Target: 5'- cGGCCGccGUCCucGGGCCcgaGAGCcGCGGGg- -3' miRNA: 3'- -UUGGU--UAGGu-CCCGG---CUCG-CGUCCag -5' |
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10709 | 3' | -59.3 | NC_002794.1 | + | 3502 | 0.66 | 0.791169 |
Target: 5'- -cCCGGUcaaagaggaaCCGGuGGCCGAGUGaGGGUCc -3' miRNA: 3'- uuGGUUA----------GGUC-CCGGCUCGCgUCCAG- -5' |
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10709 | 3' | -59.3 | NC_002794.1 | + | 7165 | 0.66 | 0.791169 |
Target: 5'- uGACCAucuuuGUCCgguGGGGCUGAGgGgGGGg- -3' miRNA: 3'- -UUGGU-----UAGG---UCCCGGCUCgCgUCCag -5' |
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10709 | 3' | -59.3 | NC_002794.1 | + | 102077 | 0.66 | 0.790277 |
Target: 5'- cGACCug-CCGcGGGCCGAcgaGCcgccgcccacgguGCGGGUCa -3' miRNA: 3'- -UUGGuuaGGU-CCCGGCU---CG-------------CGUCCAG- -5' |
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10709 | 3' | -59.3 | NC_002794.1 | + | 47742 | 0.66 | 0.783091 |
Target: 5'- cGCCgGGUCCacguGGcGGCCGuggucgcggcacaacGCGCAGGUCg -3' miRNA: 3'- uUGG-UUAGG----UC-CCGGCu--------------CGCGUCCAG- -5' |
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10709 | 3' | -59.3 | NC_002794.1 | + | 147208 | 0.66 | 0.773076 |
Target: 5'- uGCCGucgggCCGGGGCCGgGGCGUuGG-Cg -3' miRNA: 3'- uUGGUua---GGUCCCGGC-UCGCGuCCaG- -5' |
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10709 | 3' | -59.3 | NC_002794.1 | + | 119947 | 0.66 | 0.773076 |
Target: 5'- cAGCCGcgCCAGcaGGUCGAGCGUgcucuGGUg -3' miRNA: 3'- -UUGGUuaGGUC--CCGGCUCGCGu----CCAg -5' |
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10709 | 3' | -59.3 | NC_002794.1 | + | 130607 | 0.66 | 0.763843 |
Target: 5'- cGCCGAUCguGGGCCGguugacGGUGUucGGcGUCg -3' miRNA: 3'- uUGGUUAGguCCCGGC------UCGCG--UC-CAG- -5' |
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10709 | 3' | -59.3 | NC_002794.1 | + | 137771 | 0.67 | 0.745051 |
Target: 5'- aGAgCAGgauGGGGUCGAgGUGCAGGUCg -3' miRNA: 3'- -UUgGUUaggUCCCGGCU-CGCGUCCAG- -5' |
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10709 | 3' | -59.3 | NC_002794.1 | + | 86532 | 0.67 | 0.745051 |
Target: 5'- cGACCGGaugcCCcGGGCCGAGC-CGGGa- -3' miRNA: 3'- -UUGGUUa---GGuCCCGGCUCGcGUCCag -5' |
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10709 | 3' | -59.3 | NC_002794.1 | + | 86364 | 0.67 | 0.745051 |
Target: 5'- cGACCGGaugcCCcGGGCCGAGC-CGGGa- -3' miRNA: 3'- -UUGGUUa---GGuCCCGGCUCGcGUCCag -5' |
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10709 | 3' | -59.3 | NC_002794.1 | + | 86172 | 0.67 | 0.745051 |
Target: 5'- cGACCGGaugcCCcGGGCCGAGC-CGGGa- -3' miRNA: 3'- -UUGGUUa---GGuCCCGGCUCGcGUCCag -5' |
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10709 | 3' | -59.3 | NC_002794.1 | + | 87030 | 0.67 | 0.735509 |
Target: 5'- cGCagGAUCCGGGGaCCG-GCGCGGcUCg -3' miRNA: 3'- uUGg-UUAGGUCCC-GGCuCGCGUCcAG- -5' |
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10709 | 3' | -59.3 | NC_002794.1 | + | 104286 | 0.67 | 0.735509 |
Target: 5'- uGCCGAacaggaUCUcGGGCCGGGUGCacuugAGGUUg -3' miRNA: 3'- uUGGUU------AGGuCCCGGCUCGCG-----UCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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