Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1088 | 5' | -54.7 | NC_000929.1 | + | 31117 | 0.73 | 0.241665 |
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Target: 5'- aCGGCACccuguccgaacGCCAGCGGGCU---GCCg -3' miRNA: 3'- gGCCGUGa----------CGGUCGUCCGAuuuUGGg -5' |
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| 1088 | 5' | -54.7 | NC_000929.1 | + | 25539 | 0.75 | 0.17855 |
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Target: 5'- -aGGUAaUGCCAGC-GGCUGugGCCCg -3' miRNA: 3'- ggCCGUgACGGUCGuCCGAUuuUGGG- -5' |
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| 1088 | 5' | -54.7 | NC_000929.1 | + | 27204 | 0.76 | 0.173114 |
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Target: 5'- aCCGGCACaGCCAGCGGGgccagcagcacauCUGcuACCg -3' miRNA: 3'- -GGCCGUGaCGGUCGUCC-------------GAUuuUGGg -5' |
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| 1088 | 5' | -54.7 | NC_000929.1 | + | 11537 | 0.76 | 0.172141 |
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Target: 5'- gUGGCGCUGCUgcucgcugaacucuGGCAGGggGAuACCCg -3' miRNA: 3'- gGCCGUGACGG--------------UCGUCCgaUUuUGGG- -5' |
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| 1088 | 5' | -54.7 | NC_000929.1 | + | 8856 | 0.81 | 0.076909 |
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Target: 5'- uCCGGCACUGCUGGCGGaGCUGAAcgauuuACUg -3' miRNA: 3'- -GGCCGUGACGGUCGUC-CGAUUU------UGGg -5' |
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| 1088 | 5' | -54.7 | NC_000929.1 | + | 24046 | 1.13 | 0.000311 |
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Target: 5'- uCCGGCACUGCCAGCAGGCUAAAACCCu -3' miRNA: 3'- -GGCCGUGACGGUCGUCCGAUUUUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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