Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1088 | 5' | -54.7 | NC_000929.1 | + | 28240 | 0.73 | 0.254968 |
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Target: 5'- gUGGCugUGCUGGCAgcccGGCUGAAuACCa -3' miRNA: 3'- gGCCGugACGGUCGU----CCGAUUU-UGGg -5' |
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| 1088 | 5' | -54.7 | NC_000929.1 | + | 28723 | 0.68 | 0.48269 |
|
Target: 5'- aCCGGCACcagaaaGCCuucgcgugguGCaAGGCUGAcgcggguuuucAGCCCg -3' miRNA: 3'- -GGCCGUGa-----CGGu---------CG-UCCGAUU-----------UUGGG- -5' |
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| 1088 | 5' | -54.7 | NC_000929.1 | + | 30025 | 0.66 | 0.59291 |
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Target: 5'- -gGaGCACUGCCGGUucugGGGCgugcGCCg -3' miRNA: 3'- ggC-CGUGACGGUCG----UCCGauuuUGGg -5' |
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| 1088 | 5' | -54.7 | NC_000929.1 | + | 30119 | 0.66 | 0.625918 |
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Target: 5'- gCCGGUACacGCUGGCAGcguGCUGAuggugugguuuacAGCCUg -3' miRNA: 3'- -GGCCGUGa-CGGUCGUC---CGAUU-------------UUGGG- -5' |
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| 1088 | 5' | -54.7 | NC_000929.1 | + | 30907 | 0.66 | 0.627059 |
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Target: 5'- aCGGCcuuucugcaauuACUGCCGucaGGGCUGccuggaauaaAAGCCCc -3' miRNA: 3'- gGCCG------------UGACGGUcg-UCCGAU----------UUUGGG- -5' |
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| 1088 | 5' | -54.7 | NC_000929.1 | + | 31117 | 0.73 | 0.241665 |
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Target: 5'- aCGGCACccuguccgaacGCCAGCGGGCU---GCCg -3' miRNA: 3'- gGCCGUGa----------CGGUCGUCCGAuuuUGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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