Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1088 | 5' | -54.7 | NC_000929.1 | + | 18172 | 0.7 | 0.402089 |
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Target: 5'- aCgGGCACUGaaaugaCCGGCAGGCaauAGCUg -3' miRNA: 3'- -GgCCGUGAC------GGUCGUCCGauuUUGGg -5' |
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| 1088 | 5' | -54.7 | NC_000929.1 | + | 24096 | 0.7 | 0.399235 |
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Target: 5'- gCUGGCAgUGCCggaaaaagcgggacAGCGGGagagacAAGGCCCa -3' miRNA: 3'- -GGCCGUgACGG--------------UCGUCCga----UUUUGGG- -5' |
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| 1088 | 5' | -54.7 | NC_000929.1 | + | 25583 | 0.7 | 0.392626 |
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Target: 5'- aCCGGUAC-GCCGGguGGggGuAAugCCa -3' miRNA: 3'- -GGCCGUGaCGGUCguCCgaU-UUugGG- -5' |
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| 1088 | 5' | -54.7 | NC_000929.1 | + | 24736 | 0.71 | 0.347538 |
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Target: 5'- aCCGuaACcgGUCAGCAGGUUGGcAUCCg -3' miRNA: 3'- -GGCcgUGa-CGGUCGUCCGAUUuUGGG- -5' |
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| 1088 | 5' | -54.7 | NC_000929.1 | + | 28240 | 0.73 | 0.254968 |
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Target: 5'- gUGGCugUGCUGGCAgcccGGCUGAAuACCa -3' miRNA: 3'- gGCCGugACGGUCGU----CCGAUUU-UGGg -5' |
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| 1088 | 5' | -54.7 | NC_000929.1 | + | 25539 | 0.75 | 0.17855 |
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Target: 5'- -aGGUAaUGCCAGC-GGCUGugGCCCg -3' miRNA: 3'- ggCCGUgACGGUCGuCCGAUuuUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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