Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1088 | 5' | -54.7 | NC_000929.1 | + | 18929 | 0.68 | 0.514884 |
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Target: 5'- gCGGCACUGUCGGCAcaGCUugagaaAAAACagCCa -3' miRNA: 3'- gGCCGUGACGGUCGUc-CGA------UUUUG--GG- -5' |
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| 1088 | 5' | -54.7 | NC_000929.1 | + | 11028 | 0.68 | 0.504051 |
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Target: 5'- -gGGUugUGgCA-CAGGCUGGAcagcACCCg -3' miRNA: 3'- ggCCGugACgGUcGUCCGAUUU----UGGG- -5' |
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| 1088 | 5' | -54.7 | NC_000929.1 | + | 6969 | 0.68 | 0.493318 |
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Target: 5'- cCCGcaGCACUGCUGGCGGG----GACCa -3' miRNA: 3'- -GGC--CGUGACGGUCGUCCgauuUUGGg -5' |
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| 1088 | 5' | -54.7 | NC_000929.1 | + | 19558 | 0.68 | 0.472174 |
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Target: 5'- aUGGCACgGgCAGCGcGGUUAAuACCUc -3' miRNA: 3'- gGCCGUGaCgGUCGU-CCGAUUuUGGG- -5' |
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| 1088 | 5' | -54.7 | NC_000929.1 | + | 14818 | 0.69 | 0.461775 |
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Target: 5'- uCCGGCACgugaUGCCAGCgcacAGGagaaaaAAGACgCCg -3' miRNA: 3'- -GGCCGUG----ACGGUCG----UCCga----UUUUG-GG- -5' |
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| 1088 | 5' | -54.7 | NC_000929.1 | + | 24046 | 1.13 | 0.000311 |
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Target: 5'- uCCGGCACUGCCAGCAGGCUAAAACCCu -3' miRNA: 3'- -GGCCGUGACGGUCGUCCGAUUUUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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