Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11185 | 5' | -46.9 | NC_002816.1 | + | 80871 | 0.67 | 0.999304 |
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Target: 5'- aCACACccaacauuGUUUugUcg-GACAAAGUGGCg -3' miRNA: 3'- -GUGUG--------CAAGugGucaUUGUUUCACCG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 11229 | 0.66 | 0.999556 |
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Target: 5'- -cCACG-UCAUCAGUGAgAAGGUGu- -3' miRNA: 3'- guGUGCaAGUGGUCAUUgUUUCACcg -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 46993 | 0.66 | 0.999556 |
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Target: 5'- uCACGCGUUUucUCAGcGAgGuuGUGGCg -3' miRNA: 3'- -GUGUGCAAGu-GGUCaUUgUuuCACCG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 59299 | 0.66 | 0.999596 |
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Target: 5'- gGCugGauugUUGCCAGUuugacuugaacgcggAGCGAAGUGuGCg -3' miRNA: 3'- gUGugCa---AGUGGUCA---------------UUGUUUCAC-CG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 3219 | 0.66 | 0.999725 |
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Target: 5'- aCACAaGUUUaauguGCCAcauacAACAAGGUGGCg -3' miRNA: 3'- -GUGUgCAAG-----UGGUca---UUGUUUCACCG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 1825 | 0.66 | 0.999725 |
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Target: 5'- gACAuCGUUCgACUGGUgggcgGugGGAGUGGUg -3' miRNA: 3'- gUGU-GCAAG-UGGUCA-----UugUUUCACCG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 121957 | 0.66 | 0.999775 |
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Target: 5'- gCACACGgcgcggCAaugggguaaucaCGGUAACGuuGUGGCc -3' miRNA: 3'- -GUGUGCaa----GUg-----------GUCAUUGUuuCACCG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 32135 | 0.66 | 0.999786 |
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Target: 5'- aUACAUGUUCGaCAGUuuugucAGCAcuuuuGAGUGGUc -3' miRNA: 3'- -GUGUGCAAGUgGUCA------UUGU-----UUCACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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