Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11185 | 5' | -46.9 | NC_002816.1 | + | 77412 | 0.69 | 0.994604 |
|
Target: 5'- aCACACug-CACaauuGGUGACGA-GUGGCg -3' miRNA: 3'- -GUGUGcaaGUGg---UCAUUGUUuCACCG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 80871 | 0.67 | 0.999304 |
|
Target: 5'- aCACACccaacauuGUUUugUcg-GACAAAGUGGCg -3' miRNA: 3'- -GUGUG--------CAAGugGucaUUGUUUCACCG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 83533 | 0.83 | 0.512137 |
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Target: 5'- gACACGggCGCCAGUGuguGCGAAGggGGCa -3' miRNA: 3'- gUGUGCaaGUGGUCAU---UGUUUCa-CCG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 83737 | 1.14 | 0.008859 |
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Target: 5'- gCACACGUUCACCAGUAACAAAGUGGCg -3' miRNA: 3'- -GUGUGCAAGUGGUCAUUGUUUCACCG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 85010 | 0.69 | 0.993702 |
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Target: 5'- gGCACGguguuagUAUCGGgaaaGACGAGGUGGUg -3' miRNA: 3'- gUGUGCaa-----GUGGUCa---UUGUUUCACCG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 105421 | 0.67 | 0.999136 |
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Target: 5'- gCACAgUGUUCACUAGUG---GGGUGcGCu -3' miRNA: 3'- -GUGU-GCAAGUGGUCAUuguUUCAC-CG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 110518 | 0.76 | 0.863999 |
|
Target: 5'- gCGCugGguaucccgUCGCCGGUGuuGAAGUGGUg -3' miRNA: 3'- -GUGugCa-------AGUGGUCAUugUUUCACCG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 121957 | 0.66 | 0.999775 |
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Target: 5'- gCACACGgcgcggCAaugggguaaucaCGGUAACGuuGUGGCc -3' miRNA: 3'- -GUGUGCaa----GUg-----------GUCAUUGUuuCACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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