Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11185 | 5' | -46.9 | NC_002816.1 | + | 761 | 0.69 | 0.996096 |
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Target: 5'- --aACGUaCACCAGUGGCGguagAAGUagGGCc -3' miRNA: 3'- gugUGCAaGUGGUCAUUGU----UUCA--CCG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 21231 | 0.68 | 0.997232 |
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Target: 5'- --aGgGUUUGCCAGagGugAAGGUGGCu -3' miRNA: 3'- gugUgCAAGUGGUCa-UugUUUCACCG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 4016 | 0.67 | 0.998935 |
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Target: 5'- aGCAUG-UCACCGacuacaugGACAAGGUGaGCa -3' miRNA: 3'- gUGUGCaAGUGGUca------UUGUUUCAC-CG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 105421 | 0.67 | 0.999136 |
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Target: 5'- gCACAgUGUUCACUAGUG---GGGUGcGCu -3' miRNA: 3'- -GUGU-GCAAGUGGUCAUuguUUCAC-CG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 80871 | 0.67 | 0.999304 |
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Target: 5'- aCACACccaacauuGUUUugUcg-GACAAAGUGGCg -3' miRNA: 3'- -GUGUG--------CAAGugGucaUUGUUUCACCG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 11229 | 0.66 | 0.999556 |
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Target: 5'- -cCACG-UCAUCAGUGAgAAGGUGu- -3' miRNA: 3'- guGUGCaAGUGGUCAUUgUUUCACcg -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 59299 | 0.66 | 0.999596 |
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Target: 5'- gGCugGauugUUGCCAGUuugacuugaacgcggAGCGAAGUGuGCg -3' miRNA: 3'- gUGugCa---AGUGGUCA---------------UUGUUUCAC-CG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 83737 | 1.14 | 0.008859 |
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Target: 5'- gCACACGUUCACCAGUAACAAAGUGGCg -3' miRNA: 3'- -GUGUGCAAGUGGUCAUUGUUUCACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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