Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11185 | 5' | -46.9 | NC_002816.1 | + | 74452 | 0.68 | 0.996704 |
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Target: 5'- gCGCuGCGUUCACCcc--GCucAGUGGCu -3' miRNA: 3'- -GUG-UGCAAGUGGucauUGuuUCACCG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 21668 | 0.68 | 0.997232 |
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Target: 5'- aCACACGUaaCACgCAGUGGCAugcaUGGUg -3' miRNA: 3'- -GUGUGCAa-GUG-GUCAUUGUuuc-ACCG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 21417 | 0.68 | 0.997687 |
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Target: 5'- uCACA-GUagUACCcGUAACAAuuGGUGGCg -3' miRNA: 3'- -GUGUgCAa-GUGGuCAUUGUU--UCACCG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 26767 | 0.68 | 0.998413 |
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Target: 5'- aACACG--CGCUAGgcAcCAAGGUGGUa -3' miRNA: 3'- gUGUGCaaGUGGUCauU-GUUUCACCG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 41104 | 0.67 | 0.998696 |
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Target: 5'- uGCGCGaaUUCaucuACCAGUugGGCAGaacuauAGUGGCg -3' miRNA: 3'- gUGUGC--AAG----UGGUCA--UUGUU------UCACCG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 46993 | 0.66 | 0.999556 |
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Target: 5'- uCACGCGUUUucUCAGcGAgGuuGUGGCg -3' miRNA: 3'- -GUGUGCAAGu-GGUCaUUgUuuCACCG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 3219 | 0.66 | 0.999725 |
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Target: 5'- aCACAaGUUUaauguGCCAcauacAACAAGGUGGCg -3' miRNA: 3'- -GUGUgCAAG-----UGGUca---UUGUUUCACCG- -5' |
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| 11185 | 5' | -46.9 | NC_002816.1 | + | 110518 | 0.76 | 0.863999 |
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Target: 5'- gCGCugGguaucccgUCGCCGGUGuuGAAGUGGUg -3' miRNA: 3'- -GUGugCa-------AGUGGUCAUugUUUCACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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