Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11599 | 5' | -60.4 | NC_003085.1 | + | 3636 | 0.7 | 0.243327 |
Target: 5'- uGCACCGUCGaUGCUCGguggcagaCGGaGugGCa- -3' miRNA: 3'- -CGUGGCGGC-ACGAGCa-------GCC-CugCGag -5' |
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11599 | 5' | -60.4 | NC_003085.1 | + | 11085 | 0.7 | 0.239682 |
Target: 5'- uGCACCGCCaggagucugcgccgaGUGacuucuccgccUUCGUUGGGGCGCg- -3' miRNA: 3'- -CGUGGCGG---------------CAC-----------GAGCAGCCCUGCGag -5' |
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11599 | 5' | -60.4 | NC_003085.1 | + | 40630 | 0.71 | 0.214329 |
Target: 5'- cCGCUGCCGUcCUCGUCGcccuGACGCUg -3' miRNA: 3'- cGUGGCGGCAcGAGCAGCc---CUGCGAg -5' |
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11599 | 5' | -60.4 | NC_003085.1 | + | 39480 | 0.72 | 0.17412 |
Target: 5'- -uGCCGaCGUGCgCGUCGaGGCGCUCg -3' miRNA: 3'- cgUGGCgGCACGaGCAGCcCUGCGAG- -5' |
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11599 | 5' | -60.4 | NC_003085.1 | + | 27839 | 0.74 | 0.133401 |
Target: 5'- uGCGCCaugGCCGU-CUCGcgCGGGuCGCUCg -3' miRNA: 3'- -CGUGG---CGGCAcGAGCa-GCCCuGCGAG- -5' |
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11599 | 5' | -60.4 | NC_003085.1 | + | 7287 | 0.74 | 0.123009 |
Target: 5'- cGCACCGCCGccaaGCUCGaCGGGAaGuCUCc -3' miRNA: 3'- -CGUGGCGGCa---CGAGCaGCCCUgC-GAG- -5' |
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11599 | 5' | -60.4 | NC_003085.1 | + | 38614 | 1.11 | 0.000217 |
Target: 5'- uGCACCGCCGUGCUCGUCGGGACGCUCa -3' miRNA: 3'- -CGUGGCGGCACGAGCAGCCCUGCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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