Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11599 | 5' | -60.4 | NC_003085.1 | + | 17815 | 0.68 | 0.3499 |
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Target: 5'- -uGCCGCCGaGCa-GUCGGGcuGgGCUCa -3' miRNA: 3'- cgUGGCGGCaCGagCAGCCC--UgCGAG- -5' |
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| 11599 | 5' | -60.4 | NC_003085.1 | + | 41939 | 0.68 | 0.3499 |
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Target: 5'- gGC-CCGCgCGUcCUCGUCGGuGAuguccugcaacCGCUCg -3' miRNA: 3'- -CGuGGCG-GCAcGAGCAGCC-CU-----------GCGAG- -5' |
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| 11599 | 5' | -60.4 | NC_003085.1 | + | 24257 | 0.67 | 0.374836 |
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Target: 5'- gGUACCaGCCGUacuGCUCGgCGacguuGGCGCUCa -3' miRNA: 3'- -CGUGG-CGGCA---CGAGCaGCc----CUGCGAG- -5' |
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| 11599 | 5' | -60.4 | NC_003085.1 | + | 8637 | 0.67 | 0.374836 |
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Target: 5'- -gGCCGCCGUcagguacagGC-CGgcCGGGACGCg- -3' miRNA: 3'- cgUGGCGGCA---------CGaGCa-GCCCUGCGag -5' |
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| 11599 | 5' | -60.4 | NC_003085.1 | + | 28950 | 0.67 | 0.400946 |
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Target: 5'- uGCGCggaGUCGcGCUCGUCGcGGuacCGCUUg -3' miRNA: 3'- -CGUGg--CGGCaCGAGCAGC-CCu--GCGAG- -5' |
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| 11599 | 5' | -60.4 | NC_003085.1 | + | 32690 | 0.66 | 0.45649 |
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Target: 5'- cCACUGCUGgcGCgUCGUCuGGuaGCGCUCg -3' miRNA: 3'- cGUGGCGGCa-CG-AGCAGcCC--UGCGAG- -5' |
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| 11599 | 5' | -60.4 | NC_003085.1 | + | 27839 | 0.74 | 0.133401 |
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Target: 5'- uGCGCCaugGCCGU-CUCGcgCGGGuCGCUCg -3' miRNA: 3'- -CGUGG---CGGCAcGAGCa-GCCCuGCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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