Results 1 - 20 of 53 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11654 | 5' | -50.1 | NC_003102.1 | + | 137192 | 0.66 | 0.99058 |
Target: 5'- gAACgUGGCCaACUUUUCGUGuacauGUUAAa -3' miRNA: 3'- -UUGgACCGGcUGAAAAGCAC-----UAGUUa -5' |
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11654 | 5' | -50.1 | NC_003102.1 | + | 130 | 0.67 | 0.979811 |
Target: 5'- cGCgaGGCCGACgag-CGUGAgcUCGAUc -3' miRNA: 3'- uUGgaCCGGCUGaaaaGCACU--AGUUA- -5' |
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11654 | 5' | -50.1 | NC_003102.1 | + | 42728 | 0.67 | 0.979811 |
Target: 5'- cAACUUGuCUGACUUUUCGUGAaCAu- -3' miRNA: 3'- -UUGGACcGGCUGAAAAGCACUaGUua -5' |
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11654 | 5' | -50.1 | NC_003102.1 | + | 54789 | 0.68 | 0.974708 |
Target: 5'- gAACCUGGCUGACUUUUUa-GAUUu-- -3' miRNA: 3'- -UUGGACCGGCUGAAAAGcaCUAGuua -5' |
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11654 | 5' | -50.1 | NC_003102.1 | + | 54314 | 0.68 | 0.965382 |
Target: 5'- cAACUUGGCCGACUUUUcaaaguauaaaCGUG-UUAAa -3' miRNA: 3'- -UUGGACCGGCUGAAAA-----------GCACuAGUUa -5' |
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11654 | 5' | -50.1 | NC_003102.1 | + | 61601 | 0.69 | 0.953871 |
Target: 5'- gAACCUGGCaGACUUUUCGUa------ -3' miRNA: 3'- -UUGGACCGgCUGAAAAGCAcuaguua -5' |
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11654 | 5' | -50.1 | NC_003102.1 | + | 89508 | 0.69 | 0.940019 |
Target: 5'- gAACgUGGCUGACUUUUCGUaGAa---- -3' miRNA: 3'- -UUGgACCGGCUGAAAAGCA-CUaguua -5' |
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11654 | 5' | -50.1 | NC_003102.1 | + | 112472 | 0.69 | 0.940019 |
Target: 5'- gAACCUGGCUGACUUUUCa-------- -3' miRNA: 3'- -UUGGACCGGCUGAAAAGcacuaguua -5' |
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11654 | 5' | -50.1 | NC_003102.1 | + | 104287 | 0.7 | 0.929438 |
Target: 5'- uAACCUGGuuGACUUUUCGauaagccAUCGAc -3' miRNA: 3'- -UUGGACCggCUGAAAAGCac-----UAGUUa -5' |
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11654 | 5' | -50.1 | NC_003102.1 | + | 96498 | 0.7 | 0.923738 |
Target: 5'- uAACCUGGCCGACaaucUGaUGAUCGGc -3' miRNA: 3'- -UUGGACCGGCUGaaaaGC-ACUAGUUa -5' |
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11654 | 5' | -50.1 | NC_003102.1 | + | 42596 | 0.7 | 0.911519 |
Target: 5'- gAACCUGGCCGACcgcaaacUUGUGAuauuuUCAAa -3' miRNA: 3'- -UUGGACCGGCUGaaa----AGCACU-----AGUUa -5' |
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11654 | 5' | -50.1 | NC_003102.1 | + | 54594 | 0.71 | 0.89822 |
Target: 5'- gAACCUGGCCGACUUUUUuuUGAc---- -3' miRNA: 3'- -UUGGACCGGCUGAAAAGc-ACUaguua -5' |
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11654 | 5' | -50.1 | NC_003102.1 | + | 26326 | 0.71 | 0.89822 |
Target: 5'- gAAUUUGGCCGACUUUUUGgucAUCGAc -3' miRNA: 3'- -UUGGACCGGCUGAAAAGCac-UAGUUa -5' |
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11654 | 5' | -50.1 | NC_003102.1 | + | 26362 | 0.72 | 0.843628 |
Target: 5'- gAACgUGGCCGACUUUUCGaGAaaGAUg -3' miRNA: 3'- -UUGgACCGGCUGAAAAGCaCUagUUA- -5' |
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11654 | 5' | -50.1 | NC_003102.1 | + | 97896 | 0.72 | 0.834891 |
Target: 5'- gAACgUGGCCGACUUUUCGUa--CAAa -3' miRNA: 3'- -UUGgACCGGCUGAAAAGCAcuaGUUa -5' |
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11654 | 5' | -50.1 | NC_003102.1 | + | 97953 | 0.72 | 0.834891 |
Target: 5'- gAACgUGGCCGACUUUUCGUa--CAAa -3' miRNA: 3'- -UUGgACCGGCUGAAAAGCAcuaGUUa -5' |
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11654 | 5' | -50.1 | NC_003102.1 | + | 98010 | 0.72 | 0.834891 |
Target: 5'- gAACgUGGCCGACUUUUCGUa--CAAa -3' miRNA: 3'- -UUGgACCGGCUGAAAAGCAcuaGUUa -5' |
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11654 | 5' | -50.1 | NC_003102.1 | + | 103657 | 0.72 | 0.834891 |
Target: 5'- gAACCUGGCCGACUcUUCauaGAUUGAa -3' miRNA: 3'- -UUGGACCGGCUGAaAAGca-CUAGUUa -5' |
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11654 | 5' | -50.1 | NC_003102.1 | + | 133275 | 0.73 | 0.816794 |
Target: 5'- cAACCUGGCCGGCUUUUa--GAUUAc- -3' miRNA: 3'- -UUGGACCGGCUGAAAAgcaCUAGUua -5' |
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11654 | 5' | -50.1 | NC_003102.1 | + | 112653 | 0.73 | 0.816794 |
Target: 5'- gAACCUGuCUGACUUUUCGUGAaCAu- -3' miRNA: 3'- -UUGGACcGGCUGAAAAGCACUaGUua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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