Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11670 | 5' | -54.3 | NC_003102.1 | + | 16418 | 0.66 | 0.959412 |
Target: 5'- aCGaCGGGCGUcugguuGAUCGCCGcGAuuCuGCCg -3' miRNA: 3'- aGC-GCCUGCG------CUAGCGGU-CUuuG-UGG- -5' |
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11670 | 5' | -54.3 | NC_003102.1 | + | 75053 | 0.66 | 0.959412 |
Target: 5'- cUCGaUGGACuGuUGAUCGCCGacgcuaccgacGAGAUGCCa -3' miRNA: 3'- -AGC-GCCUG-C-GCUAGCGGU-----------CUUUGUGG- -5' |
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11670 | 5' | -54.3 | NC_003102.1 | + | 118368 | 0.66 | 0.959412 |
Target: 5'- cUCGCaacuGGACGcCGAUCagaacGCaaugauGAAACGCCa -3' miRNA: 3'- -AGCG----CCUGC-GCUAG-----CGgu----CUUUGUGG- -5' |
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11670 | 5' | -54.3 | NC_003102.1 | + | 90322 | 0.66 | 0.959412 |
Target: 5'- --uUGGACGCGG-CGCCgaacaAGAcgaugGGCGCCg -3' miRNA: 3'- agcGCCUGCGCUaGCGG-----UCU-----UUGUGG- -5' |
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11670 | 5' | -54.3 | NC_003102.1 | + | 135677 | 0.66 | 0.959412 |
Target: 5'- aUCGaugGGAuCGCcgucGUCGCCAGAAAUugaauuACCg -3' miRNA: 3'- -AGCg--CCU-GCGc---UAGCGGUCUUUG------UGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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