miRNA: 3'- agCGcCUGCGcUAGCGGUC---UUUGUGg -5'
miRNA: 3'- agCGC-CUGCGCUaGCGGuCUUUGUGG- -5'
miRNA: 3'- -AGCGcCU--GCGCuAGCGGUCUuugUGG- -5'
miRNA: 3'- agCGCC--UG-CGCUAGCGgUCUU----UGUGG- -5'
miRNA: 3'- -AGCg--CCU-GCGc---UAGCGGUCUUUG------UGG- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11670 | 5' | -54.3 | NC_003102.1 | + | 130741 | 0.69 | 0.871124 |
|
Target: 5'- -aGC-GACGCcGUCGCCAGcauGAACACg -3' miRNA: 3'- agCGcCUGCGcUAGCGGUC---UUUGUGg -5' |
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| 11670 | 5' | -54.3 | NC_003102.1 | + | 131513 | 0.67 | 0.911343 |
|
Target: 5'- -aGUGcGAUGCGA-CGCCcGAGACAUUa -3' miRNA: 3'- agCGC-CUGCGCUaGCGGuCUUUGUGG- -5' |
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| 11670 | 5' | -54.3 | NC_003102.1 | + | 133527 | 0.67 | 0.928274 |
|
Target: 5'- aUCGCuGAucCGCG-UCGCCGGAucgACCg -3' miRNA: 3'- -AGCGcCU--GCGCuAGCGGUCUuugUGG- -5' |
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| 11670 | 5' | -54.3 | NC_003102.1 | + | 134382 | 0.7 | 0.823173 |
|
Target: 5'- -gGCGGcgGCgGCGGUCGCaAGAAuuugGCGCCg -3' miRNA: 3'- agCGCC--UG-CGCUAGCGgUCUU----UGUGG- -5' |
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| 11670 | 5' | -54.3 | NC_003102.1 | + | 135677 | 0.66 | 0.959412 |
|
Target: 5'- aUCGaugGGAuCGCcgucGUCGCCAGAAAUugaauuACCg -3' miRNA: 3'- -AGCg--CCU-GCGc---UAGCGGUCUUUG------UGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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