miRNA: 3'- aGCGCCUGCgcUAGCGGu-----CUUUGUGg -5'
miRNA: 3'- -AGCGCc------------UGCGCUAGCGGUcuUuGUGG- -5'
miRNA: 3'- aGCGCCUGcGCuAGCGGUCuuuGUGG- -5'
miRNA: 3'- agCGc--CUGCGCuaGCGGUCUUugUGG- -5'
miRNA: 3'- agCGCCugCGCUAgCG-----GUCUUUGUGg -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11670 | 5' | -54.3 | NC_003102.1 | + | 16334 | 0.68 | 0.892268 |
|
Target: 5'- aCGUGGACGa-GUCGCUcaagauGAAACACg -3' miRNA: 3'- aGCGCCUGCgcUAGCGGu-----CUUUGUGg -5' |
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| 11670 | 5' | -54.3 | NC_003102.1 | + | 15701 | 0.71 | 0.755581 |
|
Target: 5'- gUCGCGacgccgacgagucGCGCGAUCGCCAuuGcCACCa -3' miRNA: 3'- -AGCGCc------------UGCGCUAGCGGUcuUuGUGG- -5' |
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| 11670 | 5' | -54.3 | NC_003102.1 | + | 13838 | 0.67 | 0.917227 |
|
Target: 5'- gCGCGGACaCG-UUGCCGGu--UACCu -3' miRNA: 3'- aGCGCCUGcGCuAGCGGUCuuuGUGG- -5' |
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| 11670 | 5' | -54.3 | NC_003102.1 | + | 10046 | 0.69 | 0.848037 |
|
Target: 5'- -aGCauuGACGUGggCGCCGGAGugAUCg -3' miRNA: 3'- agCGc--CUGCGCuaGCGGUCUUugUGG- -5' |
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| 11670 | 5' | -54.3 | NC_003102.1 | + | 1593 | 0.66 | 0.951689 |
|
Target: 5'- -gGCGGugGUGGUgGUgguggCGGAGGCAUg -3' miRNA: 3'- agCGCCugCGCUAgCG-----GUCUUUGUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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