Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11670 | 5' | -54.3 | NC_003102.1 | + | 22962 | 0.66 | 0.943037 |
Target: 5'- -gGCGGACGCGuUCGUgAuGAugcguAUGCCg -3' miRNA: 3'- agCGCCUGCGCuAGCGgU-CUu----UGUGG- -5' |
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11670 | 5' | -54.3 | NC_003102.1 | + | 90026 | 0.67 | 0.938356 |
Target: 5'- -gGCGGAagaaaccguuCGCGucGUCGCaggGGAGAUGCCg -3' miRNA: 3'- agCGCCU----------GCGC--UAGCGg--UCUUUGUGG- -5' |
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11670 | 5' | -54.3 | NC_003102.1 | + | 105953 | 0.67 | 0.933436 |
Target: 5'- aUCG-GGGCGC--UCGCCAGccacguuCGCCg -3' miRNA: 3'- -AGCgCCUGCGcuAGCGGUCuuu----GUGG- -5' |
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11670 | 5' | -54.3 | NC_003102.1 | + | 92368 | 0.67 | 0.928274 |
Target: 5'- uUCGUacGGA-GCGAUCGCCAGuucGGCuuCg -3' miRNA: 3'- -AGCG--CCUgCGCUAGCGGUCu--UUGugG- -5' |
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11670 | 5' | -54.3 | NC_003102.1 | + | 72302 | 1.12 | 0.002874 |
Target: 5'- aUCGCGGACGCGAUCGCCAGAAACACCc -3' miRNA: 3'- -AGCGCCUGCGCUAGCGGUCUUUGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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