Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11670 | 5' | -54.3 | NC_003102.1 | + | 15701 | 0.71 | 0.755581 |
Target: 5'- gUCGCGacgccgacgagucGCGCGAUCGCCAuuGcCACCa -3' miRNA: 3'- -AGCGCc------------UGCGCUAGCGGUcuUuGUGG- -5' |
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11670 | 5' | -54.3 | NC_003102.1 | + | 40271 | 0.72 | 0.697204 |
Target: 5'- aCGUGGACGCGcAUCGCauagcguacgucgaCAGGGAgcuuCGCCg -3' miRNA: 3'- aGCGCCUGCGC-UAGCG--------------GUCUUU----GUGG- -5' |
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11670 | 5' | -54.3 | NC_003102.1 | + | 20139 | 0.76 | 0.497856 |
Target: 5'- cUCGUGGGCGCGGcucUCGCgucGAGCGCCa -3' miRNA: 3'- -AGCGCCUGCGCU---AGCGgucUUUGUGG- -5' |
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11670 | 5' | -54.3 | NC_003102.1 | + | 63528 | 0.77 | 0.441509 |
Target: 5'- aCGCGGACGUGAUCGguuguCCGaAAGCAUCg -3' miRNA: 3'- aGCGCCUGCGCUAGC-----GGUcUUUGUGG- -5' |
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11670 | 5' | -54.3 | NC_003102.1 | + | 72302 | 1.12 | 0.002874 |
Target: 5'- aUCGCGGACGCGAUCGCCAGAAACACCc -3' miRNA: 3'- -AGCGCCUGCGCUAGCGGUCUUUGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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