Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11687 | 3' | -54.2 | NC_003102.1 | + | 121639 | 0.67 | 0.90029 |
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Target: 5'- -uUUCGCCGCCGACAucuauuauagucgagGgaaaCACGCa- -3' miRNA: 3'- gcAAGCGGCGGCUGUua-------------Ca---GUGCGau -5' |
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| 11687 | 3' | -54.2 | NC_003102.1 | + | 15896 | 0.67 | 0.914049 |
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Target: 5'- uGUUCGUgGCCGAuuCGAUGguggcaauggcgaUCGCGCg- -3' miRNA: 3'- gCAAGCGgCGGCU--GUUAC-------------AGUGCGau -5' |
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| 11687 | 3' | -54.2 | NC_003102.1 | + | 35048 | 0.66 | 0.931408 |
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Target: 5'- aCG-UCGCCGUCGACGAUGagAC-Ca- -3' miRNA: 3'- -GCaAGCGGCGGCUGUUACagUGcGau -5' |
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| 11687 | 3' | -54.2 | NC_003102.1 | + | 115050 | 0.66 | 0.931408 |
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Target: 5'- cCGUU-GCCGUauCGAC-AUGUgACGCUAa -3' miRNA: 3'- -GCAAgCGGCG--GCUGuUACAgUGCGAU- -5' |
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| 11687 | 3' | -54.2 | NC_003102.1 | + | 50037 | 0.66 | 0.945938 |
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Target: 5'- uCGggCGUccuCGCCGACGGaccggccacUGUCACGUa- -3' miRNA: 3'- -GCaaGCG---GCGGCUGUU---------ACAGUGCGau -5' |
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| 11687 | 3' | -54.2 | NC_003102.1 | + | 14804 | 0.66 | 0.945938 |
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Target: 5'- aCGaUCGCaCGCCGGCGuuuuacgaacugAUGgcgCugGCUGu -3' miRNA: 3'- -GCaAGCG-GCGGCUGU------------UACa--GugCGAU- -5' |
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| 11687 | 3' | -54.2 | NC_003102.1 | + | 78598 | 0.66 | 0.949868 |
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Target: 5'- cCGUcgUCGCCGCCGuCAucuUGUaccaggaUACGCg- -3' miRNA: 3'- -GCA--AGCGGCGGCuGUu--ACA-------GUGCGau -5' |
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| 11687 | 3' | -54.2 | NC_003102.1 | + | 63236 | 0.71 | 0.752248 |
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Target: 5'- cCGUgcCGCCGCCGAgAucGUGUCuCGCg- -3' miRNA: 3'- -GCAa-GCGGCGGCUgU--UACAGuGCGau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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