Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11687 | 3' | -54.2 | NC_003102.1 | + | 71744 | 0.66 | 0.926069 |
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Target: 5'- uGUccUUGUCGCCGACGGcgacCACGCUGc -3' miRNA: 3'- gCA--AGCGGCGGCUGUUaca-GUGCGAU- -5' |
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| 11687 | 3' | -54.2 | NC_003102.1 | + | 77596 | 0.73 | 0.587783 |
|
Target: 5'- cCGUUCGUCGUUaugGACAcUGUCGCGCa- -3' miRNA: 3'- -GCAAGCGGCGG---CUGUuACAGUGCGau -5' |
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| 11687 | 3' | -54.2 | NC_003102.1 | + | 78598 | 0.66 | 0.949868 |
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Target: 5'- cCGUcgUCGCCGCCGuCAucuUGUaccaggaUACGCg- -3' miRNA: 3'- -GCA--AGCGGCGGCuGUu--ACA-------GUGCGau -5' |
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| 11687 | 3' | -54.2 | NC_003102.1 | + | 92977 | 0.68 | 0.874537 |
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Target: 5'- ---cCGCCGCCGAUAccGUCuuugACGCg- -3' miRNA: 3'- gcaaGCGGCGGCUGUuaCAG----UGCGau -5' |
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| 11687 | 3' | -54.2 | NC_003102.1 | + | 100139 | 0.68 | 0.859317 |
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Target: 5'- --aUCGUgGUCGGCGAcgGUCGCGCa- -3' miRNA: 3'- gcaAGCGgCGGCUGUUa-CAGUGCGau -5' |
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| 11687 | 3' | -54.2 | NC_003102.1 | + | 115050 | 0.66 | 0.931408 |
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Target: 5'- cCGUU-GCCGUauCGAC-AUGUgACGCUAa -3' miRNA: 3'- -GCAAgCGGCG--GCUGuUACAgUGCGAU- -5' |
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| 11687 | 3' | -54.2 | NC_003102.1 | + | 121639 | 0.67 | 0.90029 |
|
Target: 5'- -uUUCGCCGCCGACAucuauuauagucgagGgaaaCACGCa- -3' miRNA: 3'- gcAAGCGGCGGCUGUua-------------Ca---GUGCGau -5' |
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| 11687 | 3' | -54.2 | NC_003102.1 | + | 133742 | 0.68 | 0.881812 |
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Target: 5'- gCGUcgcaGCCGCCG-UAGUGUCGcCGCa- -3' miRNA: 3'- -GCAag--CGGCGGCuGUUACAGU-GCGau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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