miRNA: 3'- -UUGggCCGaCUGAAAAGUaa-----UUGUAC- -5'
miRNA: 3'- -UUGgGCcGACUGAAAAGUAAUUGuac -5'
miRNA: 3'- -UUGggCcGACUGAAAAGUAaUUGUAC- -5'
miRNA: 3'- -UUGggCcGACUGAAAAGUAaUUGUac -5'
miRNA: 3'- -UUGggCcGACUGAAAAGUAaUUGUac -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11692 | 5' | -46.2 | NC_003102.1 | + | 54314 | 0.67 | 0.999603 |
|
Target: 5'- cAACuuGGCcGACUUUUCAaaguauaAACGUGu -3' miRNA: 3'- -UUGggCCGaCUGAAAAGUaa-----UUGUAC- -5' |
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| 11692 | 5' | -46.2 | NC_003102.1 | + | 66980 | 0.66 | 0.999891 |
|
Target: 5'- aAACgUGuCUGACUUUUCGUUAACu-- -3' miRNA: 3'- -UUGgGCcGACUGAAAAGUAAUUGuac -5' |
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| 11692 | 5' | -46.2 | NC_003102.1 | + | 89259 | 0.66 | 0.999918 |
|
Target: 5'- gAACauGuCUGACUUUUCAUaGACAUGu -3' miRNA: 3'- -UUGggCcGACUGAAAAGUAaUUGUAC- -5' |
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| 11692 | 5' | -46.2 | NC_003102.1 | + | 97764 | 0.66 | 0.999918 |
|
Target: 5'- cAACuuGuCUGACUUUUCAUaGACGg- -3' miRNA: 3'- -UUGggCcGACUGAAAAGUAaUUGUac -5' |
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| 11692 | 5' | -46.2 | NC_003102.1 | + | 97820 | 0.66 | 0.999918 |
|
Target: 5'- cAACuuGuCUGACUUUUCAUaGACGg- -3' miRNA: 3'- -UUGggCcGACUGAAAAGUAaUUGUac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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