Results 1 - 20 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12110 | 5' | -56.6 | NC_003309.1 | + | 22460 | 0.66 | 0.684716 |
Target: 5'- aUCGuGCGCgGCGAUCGcacgcugccggcGUCGaGCGCGg -3' miRNA: 3'- aGGCuCGUG-UGCUAGC------------UAGCgCGCGC- -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 36237 | 0.66 | 0.684716 |
Target: 5'- -aCGAGCACucCGAUCGccaguAUCGCcCGUGc -3' miRNA: 3'- agGCUCGUGu-GCUAGC-----UAGCGcGCGC- -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 19023 | 0.66 | 0.684716 |
Target: 5'- aUCUGcGCGC-CGucGUCGAUCGC-CGCa -3' miRNA: 3'- -AGGCuCGUGuGC--UAGCUAGCGcGCGc -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 5158 | 0.66 | 0.684716 |
Target: 5'- -gCGAGCuGCGCG-UCGc-CGCGCGCc -3' miRNA: 3'- agGCUCG-UGUGCuAGCuaGCGCGCGc -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 47024 | 0.66 | 0.663228 |
Target: 5'- aCUGcacuGCACGCGG-CGAUUG-GCGCGu -3' miRNA: 3'- aGGCu---CGUGUGCUaGCUAGCgCGCGC- -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 46119 | 0.66 | 0.663228 |
Target: 5'- --aGAGCGCACGGUCGGcaccUCGacaccgaaCGCGaCGg -3' miRNA: 3'- aggCUCGUGUGCUAGCU----AGC--------GCGC-GC- -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 15454 | 0.66 | 0.652437 |
Target: 5'- gCCGgaAGUuCACGGcgUCGAgguaGCGCGCGa -3' miRNA: 3'- aGGC--UCGuGUGCU--AGCUag--CGCGCGC- -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 12026 | 0.66 | 0.645953 |
Target: 5'- gCCGcAGUggACGCGAUCGucucGUCgaaagccugcugcccGCGCGCGg -3' miRNA: 3'- aGGC-UCG--UGUGCUAGC----UAG---------------CGCGCGC- -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 21994 | 0.66 | 0.641627 |
Target: 5'- cUCCGcGUGCuCGAugagccguugcUCGAgcCGCGCGCGg -3' miRNA: 3'- -AGGCuCGUGuGCU-----------AGCUa-GCGCGCGC- -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 35042 | 0.66 | 0.637301 |
Target: 5'- aCCGAGCGCcugugcgacgccgACGAcgugaucgauaugaUCGGUgaGCGCGCc -3' miRNA: 3'- aGGCUCGUG-------------UGCU--------------AGCUAg-CGCGCGc -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 10901 | 0.66 | 0.63081 |
Target: 5'- gCCccGC-CGCGGUCGcgGUCGCcGCGCGu -3' miRNA: 3'- aGGcuCGuGUGCUAGC--UAGCG-CGCGC- -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 5566 | 0.66 | 0.63081 |
Target: 5'- gUCUucGCGCGCGc-CGAUCGCG-GCGg -3' miRNA: 3'- -AGGcuCGUGUGCuaGCUAGCGCgCGC- -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 12377 | 0.67 | 0.619994 |
Target: 5'- aUCUGcGCACuGCGcuccuucagcuuGUUGAUCGCGuCGCGa -3' miRNA: 3'- -AGGCuCGUG-UGC------------UAGCUAGCGC-GCGC- -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 17777 | 0.67 | 0.613508 |
Target: 5'- -aCGGcGCACACgGAUCagccagccguuuuUCGCGCGCGg -3' miRNA: 3'- agGCU-CGUGUG-CUAGcu-----------AGCGCGCGC- -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 24104 | 0.67 | 0.609187 |
Target: 5'- gCCGGGaUACACGccgauAUCGGcacCGCGCGCc -3' miRNA: 3'- aGGCUC-GUGUGC-----UAGCUa--GCGCGCGc -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 16001 | 0.67 | 0.609187 |
Target: 5'- -gCGAGCGCGaauUGGUCcGUCG-GCGCGg -3' miRNA: 3'- agGCUCGUGU---GCUAGcUAGCgCGCGC- -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 11861 | 0.67 | 0.607028 |
Target: 5'- -gCGAGCucGCGCGucagauuGUCGAuguugguUCGCGCGCc -3' miRNA: 3'- agGCUCG--UGUGC-------UAGCU-------AGCGCGCGc -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 3314 | 0.67 | 0.598401 |
Target: 5'- gUCGAGCGCggaaaggcgcaGCGcGUCGAUCaGCGCuGCa -3' miRNA: 3'- aGGCUCGUG-----------UGC-UAGCUAG-CGCG-CGc -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 12649 | 0.67 | 0.598401 |
Target: 5'- -gCGAGC-CGCuGcUCGAgauagccgcgcUCGCGCGCGa -3' miRNA: 3'- agGCUCGuGUG-CuAGCU-----------AGCGCGCGC- -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 6769 | 0.67 | 0.587641 |
Target: 5'- gCCuGGUACuuGAUCGucacggCGCGCGCa -3' miRNA: 3'- aGGcUCGUGugCUAGCua----GCGCGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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