Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12110 | 5' | -56.6 | NC_003309.1 | + | 47024 | 0.66 | 0.663228 |
Target: 5'- aCUGcacuGCACGCGG-CGAUUG-GCGCGu -3' miRNA: 3'- aGGCu---CGUGUGCUaGCUAGCgCGCGC- -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 46119 | 0.66 | 0.663228 |
Target: 5'- --aGAGCGCACGGUCGGcaccUCGacaccgaaCGCGaCGg -3' miRNA: 3'- aggCUCGUGUGCUAGCU----AGC--------GCGC-GC- -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 36237 | 0.66 | 0.684716 |
Target: 5'- -aCGAGCACucCGAUCGccaguAUCGCcCGUGc -3' miRNA: 3'- agGCUCGUGu-GCUAGC-----UAGCGcGCGC- -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 19023 | 0.66 | 0.684716 |
Target: 5'- aUCUGcGCGC-CGucGUCGAUCGC-CGCa -3' miRNA: 3'- -AGGCuCGUGuGC--UAGCUAGCGcGCGc -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 5158 | 0.66 | 0.684716 |
Target: 5'- -gCGAGCuGCGCG-UCGc-CGCGCGCc -3' miRNA: 3'- agGCUCG-UGUGCuAGCuaGCGCGCGc -5' |
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12110 | 5' | -56.6 | NC_003309.1 | + | 22460 | 0.66 | 0.684716 |
Target: 5'- aUCGuGCGCgGCGAUCGcacgcugccggcGUCGaGCGCGg -3' miRNA: 3'- aGGCuCGUG-UGCUAGC------------UAGCgCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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